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Structural and functional analyses of calcium ion response factors in the mantle of Pinctada fucata
https://oist.repo.nii.ac.jp/records/949
https://oist.repo.nii.ac.jp/records/949f81fafb0-5b0d-4f4f-b22b-a58626256790
名前 / ファイル | ライセンス | アクション |
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Matsuura_Manuscript (334.6 kB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(http://creativecommons.org/Licenses/by-nc-nd/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-06-18 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Structural and functional analyses of calcium ion response factors in the mantle of Pinctada fucata | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Matsuura, Akihiro
× Matsuura, Akihiro× Yoshimura, Ko× Kintsu, Hiroyuki× Atsumi, Takashi× Tsuchihashi, Yasushi× Takeuchi, Takeshi× Satoh, Noriyuki× Negishi, Lumi× Sakuda, Shohei× Asakura, Tomiko× Imura, Yuki× Yoshimura, Etsuro× Suzuki, Michio |
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書誌情報 |
en : Journal of Structural Biology 巻 204, 号 2, p. 240-249, 発行日 2018-08-17 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The pearl oyster, Pinctada fucata, is cultured for pearl production in Japan. The shell of the pearl oyster consists of calcium carbonate and a small amount of organic matrix. Despite many studies of the shell matrix proteins, the mechanism by which calcium elements are transported from the mantle to the shell remains unclear. Investigating the molecular mechanism of calcium transportation, we prepared artificial seawater with a high concentration of calcium ions (10ASW) to induce calcification in the pearl oyster. When pearl oysters were cultured in 10ASW, unusual nanoparticles were precipitated on the surface of the nacreous layer. SDS-PAGE and 2D-PAGE analyses revealed that some calcium-sensing proteins (Sarcoplasmic Ca-binding Protein (Pf-SCP) and Pf-filamin A) might be related to the synthesis of these nanoparticles. The recombinant proteins of Pf-SCP can bind to calcium ions and accumulate nanoparticles of calcium carbonate crystals. However, transcriptomic analysis of the pearl oysters grown in 10ASW showed that the matrix protein genes in the shell did not differ before and after treatment with 10ASW. These results suggest that, despite increasing calcium transportation to the shell, treatment with a high concentration of calcium ions does not induce formation of the organic framework in the shell microstructure. These findings offer meaningful insights into the transportation of calcium elements from the mantle to the shell. | |||||
出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1047-8477 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/30125693 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.jsb.2018.08.014 | |||||
権利 | ||||||
権利情報 | © 2018 Elsevier Inc. | |||||
情報源 | ||||||
関連名称 | http://creativecommons.org/Licenses/by-nc-nd/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S1047847718302296?via%3Dihub | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |