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Target-Specific Precision of CRISPR-Mediated Genome Editing
https://oist.repo.nii.ac.jp/records/977
https://oist.repo.nii.ac.jp/records/97702380996-6ec0-4225-a6ff-750355852393
名前 / ファイル | ライセンス | アクション |
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Chakrabarti-2019-Target-Specific Precision of (2.5 MB)
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Creative Commons
Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-07-03 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Target-Specific Precision of CRISPR-Mediated Genome Editing | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Chakrabarti, Anob M.
× Chakrabarti, Anob M.× Henser-Brownhill, Tristan× Monserrat, Josep× Poetsch, Anna R.× Luscombe, Nicholas M.× Scaffidi, Paola |
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書誌情報 |
en : Molecular Cell 巻 73, 号 4, p. 699-713.e6, 発行日 2018-12-13 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The CRISPR-Cas9 system has successfully been adapted to edit the genome of various organisms. However, our ability to predict the editing outcome at specific sites is limited. Here, we examined indel profiles at over 1,000 genomic sites in human cells and uncovered general principles guiding CRISPR-mediated DNA editing. We find that precision of DNA editing (i.e., recurrence of a specific indel) varies considerably among sites, with some targets showing one highly preferred indel and others displaying numerous infrequent indels. Editing precision correlates with editing efficiency and a preference for single-nucleotide homologous insertions. Precise targets and editing outcome can be predicted based on simple rules that mainly depend on the fourth nucleotide upstream of the protospacer adjacent motif (PAM). Indel profiles are robust, but they can be influenced by chromatin features. Our findings have important implications for clinical applications of CRISPR technology and reveal general patterns of broken end joining that can provide insights into DNA repair mechanisms. | |||||
出版者 | ||||||
出版者 | Elsevier Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1097-2765 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1097-4164 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/30554945 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.molcel.2018.11.031 | |||||
権利 | ||||||
権利情報 | © 2018 The Author(s). | |||||
情報源 | ||||||
関連名称 | http://creativecommons.org/licenses/by/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.cell.com/molecular-cell/fulltext/S1097-2765(18)31001-3 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |