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Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice
https://oist.repo.nii.ac.jp/records/2374
https://oist.repo.nii.ac.jp/records/2374186b23f0-140d-484f-96c2-fd3d4c990db1
名前 / ファイル | ライセンス | アクション |
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fnins-15-741279 (4.0 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-11-19 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | voltage imaging | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | local field potentials | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | ANNINE-6 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | two-photon microscopy | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | neuroimaging | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | combined approach | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | whisker stimulation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cortex | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Cecchetto, Claudia
× Cecchetto, Claudia× Vassanelli, Stefano× Kuhn, Bernd |
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書誌情報 |
en : Frontiers in Neuroscience 巻 15, p. 741279, 発行日 2021-11-11 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Neuronal population activity, both spontaneous and sensory-evoked, generates propagating waves in cortex. However, high spatiotemporal-resolution mapping of these waves is difficult as calcium imaging, the work horse of current imaging, does not reveal subthreshold activity. Here, we present a platform combining voltage or calcium two-photon imaging with multi-channel local field potential (LFP) recordings in different layers of the barrel cortex from anesthetized and awake head-restrained mice. A chronic cranial window with access port allows injecting a viral vector expressing GCaMP6f or the voltage-sensitive dye (VSD) ANNINE-6plus, as well as entering the brain with a multi-channel neural probe. We present both average spontaneous activity and average evoked signals in response to multi-whisker air-puff stimulations. Time domain analysis shows the dependence of the evoked responses on the cortical layer and on the state of the animal, here separated into anesthetized, awake but resting, and running. The simultaneous data acquisition allows to compare the average membrane depolarization measured with ANNINE-6plus with the amplitude and shape of the LFP recordings. The calcium imaging data connects these data sets to the large existing database of this important second messenger. Interestingly, in the calcium imaging data, we found a few cells which showed a decrease in calcium concentration in response to vibrissa stimulation in awake mice. This system offers a multimodal technique to study the spatiotemporal dynamics of neuronal signals through a 3D architecture in vivo. It will provide novel insights on sensory coding, closing the gap between electrical and optical recordings. | |||||
出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1662-453X | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1662-4548 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3389/fnins.2021.741279 | |||||
権利 | ||||||
権利情報 | © 2021 Cecchetto, Vassanelli and Kuhn. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.frontiersin.org/articles/10.3389/fnins.2021.741279/full | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |