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イネ自然免疫におけるrCBPの機能
https://doi.org/10.15102/1394.00000257
https://doi.org/10.15102/1394.000002575b85eda1-befe-4a65-ba1a-fadc5d43ef72
名前 / ファイル | ライセンス | アクション |
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Abstract (94.2 kB)
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Final Exam Abstract (42.8 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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公開日 | 2018-02-21 | |||||||
タイトル | ||||||||
言語 | ja | |||||||
タイトル | イネ自然免疫におけるrCBPの機能 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | rCBP-dependent regulation in rice innate immunity | |||||||
言語 | ||||||||
言語 | eng | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
資源タイプ | doctoral thesis | |||||||
ID登録 | ||||||||
ID登録 | 10.15102/1394.00000257 | |||||||
ID登録タイプ | JaLC | |||||||
アクセス権 | ||||||||
アクセス権 | open access | |||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
著者 |
エスピナス, ニンニョ
× エスピナス, ニンニョ
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著者 (英) |
Espinas, Nino
× Espinas, Nino
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抄録 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | Plant innate immunity against bacterial attacks is a two-tiered inducible system capable of defense responses at local and systemic areas. These systems are the PTI and ETI. During infection, PTI has the ability to recognize microbial signatures upon bacterial contact, while ETI recognizes microbial protein secretions called effectors delivered inside the cell. The activation of PTI and ETI confers systemic tissues of infected plants a broad-spectrum immunity against later pathogen attacks termed systemic acquired resistance (SAR). Defense priming is an adaptive component of SAR that regulates the molecular storage of defense memory for a more effective defense response. The main aim of this work is finding a novel molecular defense signaling pathway that is controlled by acetylation at the infected (local defense) and systemic tissues (priming defense). To investigate the role of histone acetyltransferase-dependent pathway in plant immunity, I have isolated transgenic and mutant lines of rCBP, [rice Cyclic adenosine monophosphate response element-binding protein (CREB) Binding Protein], under Nipponbare cultivar background using RNAi silencing and gRNA/Cas9-mediated genome editing. Animal CBP was initially described as both transcriptional coactivator and histone acetyltransferase. The rCBP-RNAi lines with mistargeting of the other members of CBP family are characterized by massive sterility and impairment of the number of effective grains. On the other hand, the CRISPR/Cas9 mutant lines have wild-type number of effective grains. To profile the global acetylation of histone lysine-sites via rCBP, I performed mass spectrometry-based proteomics in data dependent acquisition (DDA) and parallel reaction monitoring (PRM) modes. My results showed that H3 lysine sites are possibly targeted by rCBP with very high acetylation specificity on H3K9. To implicate the role of rCBP in rice innate immunity, I conducted a pathogenesis assay with bacterial pathogen, Pseudomonas syringiae pv. oryzae (Pso). Pathogenesis assay showed that rCBP-/- mutants are resistant to Pso infection compared to segregated wild-type control. I also performed transcriptome analysis on local and systemic tissues with Pso to investigate the genome-wide effects of rCBP mutation and to identify factors with roles in both basal and systemic immune response. As a result, I have identified seven putative rCBPdependent transcriptional repressors that possibly explain the resistance phenotype of rCBP mutant lines. Overall, these data preliminary indicate that rCBP is both a positive regulator of developmental processes and a negative regulator of rice immunity. These data also suggest that rCBP may execute this dual regulatory function either through H3K9ac and/or cotranscriptional activity on target gene loci. |
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口頭試問日 | ||||||||
2018-01-05 | ||||||||
学位授与年月日 | ||||||||
学位授与年月日 | 2018-02-09 | |||||||
学位名 | ||||||||
学位名 | Doctor of Philosophy | |||||||
学位授与番号 | ||||||||
学位授与番号 | 甲第12号 | |||||||
学位授与機関 | ||||||||
学位授与機関識別子Scheme | kakenhi | |||||||
学位授与機関識別子 | 38005 | |||||||
学位授与機関名 | Okinawa Institute of Science and Technology Graduate University | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
権利 | ||||||||
権利情報 | © 2018 The Author |