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Analyzing and Tuning Ribozyme Activity by Deep Sequencing To Modulate Gene Expression Level in Mammalian Cells
https://oist.repo.nii.ac.jp/records/654
https://oist.repo.nii.ac.jp/records/6547a4076f6-6d70-450e-9b03-6726869b7e4c
名前 / ファイル | ライセンス | アクション |
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acssynbio.7b00367 (3.0 MB)
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ACS AuthorChoice
(https://pubs.acs.org /page/policy /authorchoice_termsofuse.html) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-08-14 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Analyzing and Tuning Ribozyme Activity by Deep Sequencing To Modulate Gene Expression Level in Mammalian Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Kobori, Shungo
× Kobori, Shungo× Yokobayashi, Yohei |
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書誌情報 |
en : ACS Synthetic Biology 巻 7, 号 2, p. 371-376, 発行日 2018-01-17 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Self-cleaving ribozymes, in combination with aptamers and various classes of RNAs, have been heavily engineered to create RNA devices to control gene expression. Although understanding of sequence–function relationships of ribozymes is critical for such efforts, our current knowledge of self-cleaving ribozymes is mostly limited to the results from small scale mutational studies performed under different conditions, or qualitative results of mutate-and-select experiments that may contain experimental biases. Here, we applied our strategy based on deep sequencing to comprehensively assay a large number of mutants to systematically examine the effect of the P4 stem sequence on the activity of an HDV-like ribozyme. We discovered that the ribozyme activity is highly sensitive to the sequence and the apparent stability of the varied positions. Furthermore, we demonstrated that the collection of the ribozyme variants with different activities can be used as a convenient device to fine-tune the level of gene expression in mammalian cells. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2161-5063 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/29343061 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acssynbio.7b00367 | |||||
権利 | ||||||
権利情報 | © 2018 American Chemical Society | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/abs/10.1021/acssynbio.7b00367 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |