@article{oai:oist.repo.nii.ac.jp:00001010,
 author = {Ueki, Tatsuya and Fujie, Manabu and Romaidi and Satoh, Noriyuki},
 journal = {Marine Genomics},
 month = {Nov},
 note = {Ascidians belonging to Phlebobranchia accumulate vanadium to an extraordinary degree (</= 350mM). Vanadium levels are strictly regulated and vary among ascidian species; thus, they represent well-suited models for studies on vanadium accumulation. No comprehensive study on metal accumulation and reduction in marine organisms in relation to their symbiotic bacterial communities has been published. Therefore, we performed comparative 16S rRNA amplicon sequence analyses on samples from three tissues (branchial sac, intestine, and intestinal lumen) involved in vanadium absorption, isolated from two vanadium-rich (Ascidia ahodori and Ascidia sydneiensis samea) and one vanadium-poor species (Styela plicata). For each sample, the abundance of every bacteria and an abundance value normalized to their abundance in seawater were calculated and compared. Two bacterial genera, Pseudomonas and Ralstonia, were extremely abundant in the branchial sacs of vanadium-rich ascidians. Two bacterial genera, Treponema and Borrelia, were abundant and enriched in the intestinal content of vanadium-rich ascidians. The results suggest that specific selective forces maintain the bacterial population in the three ascidian tissues examined, which contribute to successful vanadium accumulation. This study furthers the understanding of the relationship between bacterial communities and metal accumulation in marine life.},
 pages = {33--42},
 title = {Symbiotic bacteria associated with ascidian vanadium accumulation identified by 16S rRNA amplicon sequencing},
 volume = {43},
 year = {2018}
}