@article{oai:oist.repo.nii.ac.jp:00001013,
 author = {Zhang, Xinjian and Nagai, Taku and Ahammad, Rijwan Uddin and Kuroda, Keisuke and Nakamuta, Shinichi and Nakano, Takashi and Yukinawa, Naoto and Funahashi, Yasuhiro and Yamahashi, Yukie and Amano, Mutsuki and Yoshimoto, Junichiro and Yamada, Kiyofumi and Kaibuchi, Kozo},
 journal = {Neurochemistry International},
 month = {Oct},
 note = {Medium spiny neurons (MSNs) expressing dopamine D1 receptor (D1R) or D2 receptor (D2R) are major components of the striatum. Stimulation of D1R activates protein kinase A (PKA) through Golf to increase neuronal activity, while D2R stimulation inhibits PKA through Gi. Adenosine A2A receptor (A2AR) coupled to Golf is highly expressed in D2R-MSNs within the striatum. However, how dopamine and adenosine co-operatively regulate PKA activity remains largely unknown. Here, we measured Rap1gap serine 563 phosphorylation to monitor PKA activity and examined dopamine and adenosine signals in MSNs. We found that a D1R agonist increased Rap1gap phosphorylation in striatal slices and in D1R-MSNs in vivo. A2AR agonist CGS21680 increased Rap1gap phosphorylation, and pretreatment with the D2R agonist quinpirole blocked this effect in striatal slices. D2R antagonist eticlopride increased Rap1gap phosphorylation in D2R-MSNs in vivo, and the effect of eticlopride was blocked by the pretreatment with the A2AR antagonist SCH58261. These results suggest that adenosine positively regulates PKA in D2R-MSNs through A2AR, while this effect is blocked by basal dopamine in vivo. Incorporating computational model analysis, we propose that the shift from D1R-MSNs to D2R-MSNs or vice versa appears to depend predominantly on a change in dopamine concentration.},
 pages = {8--18},
 title = {Balance between dopamine and adenosine signals regulates the PKA/Rap1 pathway in striatal medium spiny neurons},
 volume = {122},
 year = {2018}
}