{"created":"2023-06-26T11:00:31.763524+00:00","id":1027,"links":{},"metadata":{"_buckets":{"deposit":"b0963e4f-499d-459f-a369-2b9cad343d20"},"_deposit":{"created_by":28,"id":"1027","owners":[28],"pid":{"revision_id":0,"type":"depid","value":"1027"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001027","sets":["6:167","6:89"]},"author_link":["5665","5662","5668","5667","5663","5666","5664"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-21","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"e46340","bibliographicVolumeNumber":"20","bibliographic_titles":[{},{"bibliographic_title":"EMBO reports","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Meshcheryakov, Vladimir A"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Schreiber, Makoto Tokoro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Villar‐Briones, Alejandro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jarrell, Kenneth F"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aizawa, Shin‐Ichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wolf, Matthias"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Many archaea swim by means of archaella. While the archaellum is similar in function to its bacterial counterpart, its structure, composition, and evolution are fundamentally different. Archaella are related to archaeal and bacterial type IV pili. Despite recent advances, our understanding of molecular processes governing archaellum assembly and stability is still incomplete. Here, we determine the structures of Methanococcus archaella by X-ray crystallography and cryo-EM. The crystal structure of Methanocaldococcus jannaschii FlaB1 is the first and only crystal structure of any archaellin to date at a resolution of 1.5 angstrom, which is put into biological context by a cryo-EM reconstruction from Methanococcus maripaludis archaella at 4 angstrom resolution created with helical single-particle analysis. Our results indicate that the archaellum is predominantly composed of FlaB1. We identify N-linked glycosylation by cryo-EM and mass spectrometry. The crystal structure reveals a highly conserved metal-binding site, which is validated by mass spectrometry and electron energy-loss spectroscopy. We show invitro that the metal-binding site, which appears to be a widespread property of archaellin, is required for filament integrity.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"EMBO Press"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/30898768","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.15252/embr.201846340","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://creativecommons.org/licenses/by-nc-nd/4.0/"}]}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.embopress.org/doi/10.15252/embr.201846340","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2019 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1469-221X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1469-3178","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-24"}],"displaytype":"detail","filename":"Meshcheryakov-2019-High-resolution archaellum.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","license_note":"Creative Commons\nAttribution-NonCommercial-NoDerivatives 4.0 International\n(https://creativecommons.org/licenses/by-nc-nd/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Meshcheryakov-2019-High-resolution archaellum","url":"https://oist.repo.nii.ac.jp/record/1027/files/Meshcheryakov-2019-High-resolution archaellum.pdf"},"version_id":"10456db7-7707-466c-b29c-dfd11219822e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"High‐resolution archaellum structure reveals a conserved metal‐binding site","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"High‐resolution archaellum structure reveals a conserved metal‐binding site","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"28","path":["89","167"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-24"},"publish_date":"2019-07-24","publish_status":"0","recid":"1027","relation_version_is_last":true,"title":["High‐resolution archaellum structure reveals a conserved metal‐binding site"],"weko_creator_id":"28","weko_shared_id":28},"updated":"2023-06-26T11:58:11.726731+00:00"}