{"created":"2023-06-26T11:00:40.484461+00:00","id":1205,"links":{},"metadata":{"_buckets":{"deposit":"9ae8801d-f278-497c-986c-35dc12a842aa"},"_deposit":{"created_by":28,"id":"1205","owners":[28],"pid":{"revision_id":0,"type":"depid","value":"1205"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001205","sets":["6:67"]},"author_link":["6672","6664","6671","6674","6665","6670","6668","6666","6673","6667","6669"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-01-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"416","bibliographicPageStart":"403","bibliographicVolumeNumber":"17","bibliographic_titles":[{},{"bibliographic_title":"RNA Biology","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mostafa, Dina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Akinori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagiya, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Tomokazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Abe, Takaya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kureha, Taku"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuba, Keiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanegae, Yumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Furuta, Yasuhide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Tadashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Toru"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Shortening of mRNA poly(A) tails (deadenylation) to trigger their decay is mediated mainly by the CCR4-NOT deadenylase complex. While four catalytic subunits (CNOT6, 6L 7, and 8) have been identified in the mammalian CCR4-NOT complex, their individual biological roles are not fully understood. In this study, we addressed the contribution of CNOT7/8 to viability of primary mouse embryonic fibroblasts (MEFs). We found that MEFs lacking CNOT7/8 expression [Cnot7/8-double knockout (dKO) MEFs] undergo cell death, whereas MEFs lacking CNOT6/6L expression (Cnot6/6l-dKO MEFs) remain viable. Co-immunoprecipitation analyses showed that CNOT6/6L are also absent from the CCR4-NOT complex in Cnot7/8-dKO MEFs. In contrast, either CNOT7 or CNOT8 still interacts with other subunits in the CCR4-NOT complex in Cnot6/6l-dKO MEFs. Exogenous expression of a CNOT7 mutant lacking catalytic activity in Cnot7/8-dKO MEFs cannot recover cell viability, even though CNOT6/6L exists to some extent in the CCR4-NOT complex, confirming that CNOT7/8 is essential for viability. Bulk poly(A) tail analysis revealed that mRNAs with longer poly(A) tails are more numerous in Cnot7/8-dKO MEFs than in Cnot6/6l-dKO MEFs. Consistent with elongated poly(A) tails, more mRNAs are upregulated and stabilized in Cnot7/8-dKO MEFs than in Cnot6/6l-dKO MEFs. Importantly, Cnot6/6l-dKO mice are viable and grow normally to adulthood. Taken together, the CNOT7/8 catalytic subunits are essential for deadenylation, which is necessary to maintain cell viability, whereas CNOT6/6L are not.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Informa UK Limited, trading as Taylor & Francis Group."}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/31924127","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1080/15476286.2019.1709747","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.tandfonline.com/doi/full/10.1080/15476286.2019.1709747?scroll=top&needAccess=true","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2020 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1547-6286","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1555-8584","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-26"}],"displaytype":"detail","filename":"2_26_2020_Essential .pdf","filesize":[{"value":"7.0 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (https://creativecommons.org/licenses/by-nc-nd/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2_26_2020_Essential ","url":"https://oist.repo.nii.ac.jp/record/1205/files/2_26_2020_Essential .pdf"},"version_id":"035ac6a4-8162-4399-92b0-c4d12f829c69"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"28","path":["67"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-02-26"},"publish_date":"2020-02-26","publish_status":"0","recid":"1205","relation_version_is_last":true,"title":["Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability"],"weko_creator_id":"28","weko_shared_id":28},"updated":"2023-06-26T11:52:12.723039+00:00"}