{"created":"2023-06-26T11:00:41.786762+00:00","id":1236,"links":{},"metadata":{"_buckets":{"deposit":"e1721ac5-0a8d-41cd-9221-e69b3211c664"},"_deposit":{"created_by":28,"id":"1236","owners":[28],"pid":{"revision_id":0,"type":"depid","value":"1236"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001236","sets":["6:64"]},"author_link":["6926","6927"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-08-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"1982","bibliographicPageStart":"1976","bibliographicVolumeNumber":"8","bibliographic_titles":[{},{"bibliographic_title":"ACS Synthetic Biology","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokobayashi, Yohei"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Synthetic riboswitches based on small molecule-responsive self-cleaving ribozymes (aptazymes) embedded in the untranslated regions (UTRs) allow chemical control of gene expression in mammalian cells. In this work, we used a guanine-responsive aptazyme to control transgene expression from a replication-incompetent vesicular stomatitis virus (VSV) vector. VSV is a nonsegmented, negative-sense, cytoplasmic RNA virus that replicates without DNA intermediates, and its applications for vaccines and oncolytic viral therapy are being explored. By inserting the guanine-activated ribozyme in the 3′ UTRs of viral genes and transgenes, GFP expression from the VSV vector in mammalian cells was repressed by as much as 26.8-fold in the presence of guanine. Furthermore, we demonstrated reversible regulation of a transgene (secreted NanoLuc) by adding and withdrawing guanine from the medium over the course of 12 days. In summary, our riboswitch-controlled VSV vector allows robust, long-term, and reversible regulation of gene expression in mammalian cells without the risk of undesirable genomic integration.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"American Chemical Society"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/31415142","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1021/acssynbio.9b00177","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://pubs.acs.org/page/policy/authorchoice_termsofuse.html"}]}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubs.acs.org/doi/abs/10.1021/acssynbio.9b00177","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2019 American Chemical Society."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2161-5063","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-06"}],"displaytype":"detail","filename":"Takahashi-2019-Reversible Gene Regulation in M.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","license_note":"ACS AuthorChoice\n(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Takahashi-2019-Reversible Gene Regulation in M","url":"https://oist.repo.nii.ac.jp/record/1236/files/Takahashi-2019-Reversible Gene Regulation in M.pdf"},"version_id":"b2994158-06f9-45c8-b6e3-f19ddbfb9bb8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Reversible Gene Regulation in Mammalian Cells Using Riboswitch-Engineered Vesicular Stomatitis Virus Vector","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Reversible Gene Regulation in Mammalian Cells Using Riboswitch-Engineered Vesicular Stomatitis Virus Vector","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"28","path":["64"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-06"},"publish_date":"2020-03-06","publish_status":"0","recid":"1236","relation_version_is_last":true,"title":["Reversible Gene Regulation in Mammalian Cells Using Riboswitch-Engineered Vesicular Stomatitis Virus Vector"],"weko_creator_id":"28","weko_shared_id":28},"updated":"2023-06-26T11:54:50.794378+00:00"}