@phdthesis{oai:oist.repo.nii.ac.jp:00001332,
 author = {Pommer, Stefan},
 month = {2020-08-29, 2020-08-29},
 note = {Striatal spiny projection neurons (SPNs) make inhibitory synaptic connections with each other via local collaterals of their main axon, forming a local lateral inhibition network. Previous studies have shown that serotonin, acting via the 5-HT1B receptor, modulates neurotransmitter release from terminals in the target nuclei of SPN projections. Despite this established function, the role of 5-HT1B receptors in lateral inhibition among SPNs remains poorly understood. Spiny neuron activity ultimately underlies basal ganglia functions and knowledge of striatal circuit activity is critical for approaching disorders like Parkinson’s disease. In this thesis, I investigated the role of the 5-HT1B receptor in modulation of lateral inhibition in the striatum. To address this issue, whole-cell patch clamp recordings were made from SPNs in acute mouse brain slices, while optically stimulating presynaptic SPNs expressing channelrhodopsin. Inhibitory postsynaptic currents (IPSCs) mediated by GABA were measured before and after application of a 5-HT1B receptor agonist. Activation of 5-HT1B receptors significantly reduced the amplitude of IPSCs in SPNs for both, direct and indirect pathway neurons. This was blocked by application of a 5-HT1B antagonist. Further analysis suggested that the effect was due to a reduced probability of presynaptic release of GABA rather than a change in quantal size. Collectively, these results show a prominent role of serotonin in modulating lateral inhibition among striatal neurons, extending existing knowledge of its role in basal ganglia function. The 5-HT1B receptor may, therefore, be a suitable target for future behavioral experiments investigating the currently unknown role of lateral inhibition in the function of the striatum.},
 school = {Okinawa Institute of Science and Technology Graduate University},
 title = {マウス線条体における有棘神経細胞間の側方抑制へのセロトニン受容体 5-HT1b の影響},
 year = {}
}