{"created":"2023-06-26T11:00:45.646977+00:00","id":1342,"links":{},"metadata":{"_buckets":{"deposit":"bc40ee21-fb70-480e-8698-4fda7d7b0c2e"},"_deposit":{"created_by":22,"id":"1342","owners":[22],"pid":{"revision_id":0,"type":"depid","value":"1342"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001342","sets":["7:200"]},"author_link":[],"item_10006_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Lisicovas, Viktoras","creatorNameLang":"en"}]}]},"item_10006_date_granted_11":{"attribute_name":"Degree Conferral Date","attribute_value_mlt":[{"subitem_dategranted":"2020-03-31"}]},"item_10006_degree_grantor_9":{"attribute_name":"Degree Conferrral Institution","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Okinawa Institute of Science and Technology Graduate University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"38005","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"Degree","attribute_value_mlt":[{"subitem_degreename":"Doctor of Philosophy"}]},"item_10006_description_7":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Developments in optical technology have produced an important shift in experimental neuroscience from electrophysiological methods for observation and stimulation to all-optical solutions. One expects this trend to continue as future developments continue to deliver, and improve upon, the original promises of the technology: 1) minimally invasive actuation and recording of neurons, and 2) a drastic increase in targets that can be treated simultaneously. Moreover, as the high costs of the technology are reduced, one may expect its larger-scale adoption in the neuroscience community. In this thesis, I describe the development and implementation of two alloptical solutions for the analysis of behavior, neuronal signaling, and stimulation, which improve on previous state-of-the-art: (1) A minimally-invasive, high signal-to-noise twophoton microscopy setup capable of simultaneous, live-imaging of a large subset of sensory neurons post activation, and (2) a low-cost tracking solution to stimulate and record behavior. I begin this thesis with a review of recent advances in optical neuroscience techniques for the study of neuronal networks with the focus on work done in Caenorhabditis elegans. Then, in chapter 2, I describe my implementation of a two-photon temporal focusing microscopy setup and show significant improvements through the use of a high power/ high pulse repetition rate excitation system, enabling live imaging with high resolution for extended periods of time. I model temperature increase during a physiological imaging scenario for different repetition rates at fixed peak intensities and find range centered around 1 MHz to be optimal. Lastly, I describe the low-cost tracking setup with the ability to stimulate and record behavior over the course of hours. The setup is capable of two-color stimulation of optogenetic proteins over the area of the behavioral arena in combination with volatile chemicals. To showcase the utility of the system, I demonstrate behavioral analysis of integration of contradictory cues. In summary, I present a set of techniques for the interrogation of neural networks from animal behavior to neuronal activity, over timescales of potentially hours and days. These techniques can be used to address a new dimension of scientific questions.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10006_dissertation_number_12":{"attribute_name":"Degree Referral Number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第46号"}]},"item_10006_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15102/1394.00001212","subitem_identifier_reg_type":"JaLC"}]},"item_10006_rights_13":{"attribute_name":"Copyright Information","attribute_value_mlt":[{"subitem_rights":"© 2020 The Author."}]},"item_10006_text_24":{"attribute_name":"Exam Date","attribute_value_mlt":[{"subitem_text_value":"2019-12-04"}]},"item_10006_version_type_18":{"attribute_name":"Version Format","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-04-02"}],"displaytype":"detail","filename":"Full Text.pdf","filesize":[{"value":"7.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Full Text","objectType":"fulltext","url":"https://oist.repo.nii.ac.jp/record/1342/files/Full Text.pdf"},"version_id":"1732bbda-86d9-401d-9120-b2303a8b485d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-04-02"}],"displaytype":"detail","filename":"Final Exam Abstract.pdf","filesize":[{"value":"42.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Final Exam Abstract","objectType":"abstract","url":"https://oist.repo.nii.ac.jp/record/1342/files/Final Exam Abstract.pdf"},"version_id":"e794b0c4-0a15-4eaa-9b28-99b0d4184af2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"行動及び神経回路ダイナミクスの長期記録における光学技術の改良","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"行動及び神経回路ダイナミクスの長期記録における光学技術の改良","subitem_title_language":"ja"},{"subitem_title":"Improvements in optical techniques to investigate the behavior and neuronal network dynamics over long timescales","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"22","path":["200"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-04-02"},"publish_date":"2020-04-02","publish_status":"0","recid":"1342","relation_version_is_last":true,"title":["行動及び神経回路ダイナミクスの長期記録における光学技術の改良"],"weko_creator_id":"22","weko_shared_id":-1},"updated":"2023-08-31T02:52:20.994589+00:00"}