{"created":"2023-06-26T11:00:49.530027+00:00","id":1416,"links":{},"metadata":{"_buckets":{"deposit":"9e6f003c-7961-48cd-8f08-7d94973057d4"},"_deposit":{"created_by":30,"id":"1416","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"1416"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001416","sets":["6:69"]},"author_link":["8611","8614","8610","8613","8609","8612"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-09-23","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageStart":"e0215185","bibliographicVolumeNumber":"14","bibliographic_titles":[{},{"bibliographic_title":"PLOS ONE","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hayakawa, Eisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Menschaert, Gerben"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Holstein, Thomas W."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Baggerman, Geert"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Schoofs, Liliane"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Neuropeptides are a class of bioactive peptides shown to be involved in various physiological processes, including metabolism, development, and reproduction. Although neuropeptide candidates have been predicted from genomic and transcriptomic data, comprehensive characterization of neuropeptide repertoires remains a challenge owing to their small size and variable sequences. De novo prediction of neuropeptides from genome or transcriptome data is difficult and usually only efficient for those peptides that have identified orthologs in other animal species. Recent peptidomics technology has enabled systematic structural identification of neuropeptides by using the combination of liquid chromatography and tandem mass spectrometry. However, reliable identification of naturally occurring peptides using a conventional tandem mass spectrometry approach, scanning spectra against a protein database, remains difficult because a large search space must be scanned due to the absence of a cleavage enzyme specification. We developed a pipeline consisting of in silico prediction of candidate neuropeptides followed by peptide-spectrum matching. This approach enables highly sensitive and reliable neuropeptide identification, as the search space for peptide-spectrum matching is highly reduced. Nematostella vectensis is a basal eumetazoan with one of the most ancient nervous systems. We scanned the Nematostella protein database for sequences displaying structural hallmarks typical of eumetazoan neuropeptide precursors, including amino- and carboxyterminal motifs and associated modifications. Peptide-spectrum matching was performed against a dataset of peptides that are cleaved in silico from these putative peptide precursors. The dozens of newly identified neuropeptides display structural similarities to bilaterian neuropeptides including tachykinin, myoinhibitory peptide, and neuromedin-U/pyrokinin, suggesting these neuropeptides occurred in the eumetazoan ancestor of all animal species.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science "}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/31545805","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1371/journal.pone.0215185","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://creativecommons.org/licenses/by/4.0/"}]}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215185","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"©2019 Hayakawa et al. "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-04-28"}],"displaytype":"detail","filename":"Hayakawa-2019-A combined strategy.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Hayakawa-2019-A combined strategy","url":"https://oist.repo.nii.ac.jp/record/1416/files/Hayakawa-2019-A combined strategy.pdf"},"version_id":"8c3915e9-50a9-4e38-88ee-4c811b92cb8e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A combined strategy of neuropeptide prediction and tandem mass spectrometry identifies evolutionarily conserved ancient neuropeptides in the sea anemone Nematostella vectensis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A combined strategy of neuropeptide prediction and tandem mass spectrometry identifies evolutionarily conserved ancient neuropeptides in the sea anemone Nematostella vectensis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["69"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-04-28"},"publish_date":"2020-04-28","publish_status":"0","recid":"1416","relation_version_is_last":true,"title":["A combined strategy of neuropeptide prediction and tandem mass spectrometry identifies evolutionarily conserved ancient neuropeptides in the sea anemone Nematostella vectensis"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-06-26T11:51:10.125275+00:00"}