{"created":"2023-06-26T11:00:52.441140+00:00","id":1468,"links":{},"metadata":{"_buckets":{"deposit":"7ae899c5-82c6-4608-b63a-e48cee5a6659"},"_deposit":{"created_by":29,"id":"1468","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"1468"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001468","sets":["6:55"]},"author_link":["8904","8902","8901","8900","8903"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-11-04","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"112883","bibliographicVolumeNumber":"209","bibliographic_titles":[{},{"bibliographic_title":"Ultramicroscopy","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Cheung, Martin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Adaniya, Hidehito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Cassidy, Cathal"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Masao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shintake, Tsumoru"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Cryo-electron microscopy (cryo-EM) has become the method of choice in the field of structural biology, owing to its unique ability to deduce structures of vitreous ice-embedded, hydrated biomolecules over a wide range of structural resolutions. As cryo-transmission electron microscopes (cryo-TEM) become increasingly specialised for high, near-atomic resolution studies, operational complexity and associated costs serve as significant barriers to widespread usability and adoptability. To facilitate the expansion and accessibility of the cryo-EM method, an efficient, user-friendly means of imaging vitreous ice-embedded biomolecules has been called for. In this study, we present a solution to this issue by integrating cryo-EM capabilities into a commercial scanning electron microscope (SEM). Utilising the principle of low-energy in-line electron holography, our newly developed hybrid microscope permits low-to-moderate resolution imaging of vitreous ice-embedded biomolecules without the need for any form of sample staining or chemical fixation. Operating at 20kV, the microscope takes advantage of the ease-of-use of SEM-based imaging and phase contrast imaging of low-energy electron holography. This study represents the first reported successful application of low-energy in-line electron holographic imaging to vitreous ice-embedded small biomolecules, the effectiveness of which is demonstrated here with three morphologically distinct specimens.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/31739191","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.ultramic.2019.112883","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://creativecommons.org/licenses/by-nc-nd/4.0/"}]}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.ultramic.2019.112883","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2019 Elsevier B.V."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0304-3991","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-04"}],"displaytype":"detail","filename":"Cheung et al- Ultramicroscopy_ OISTIR.pdf","filesize":[{"value":"13.3 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Cheung et al- Ultramicroscopy_ OISTIR","url":"https://oist.repo.nii.ac.jp/record/1468/files/Cheung et al- Ultramicroscopy_ OISTIR.pdf"},"version_id":"9273b93e-2cc5-4721-a6e8-606f03e3b256"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Low-energy in-line electron holographic imaging of vitreous ice-embedded small biomolecules using a modified scanning electron microscope","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Low-energy in-line electron holographic imaging of vitreous ice-embedded small biomolecules using a modified scanning electron microscope","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"29","path":["55"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-05-11"},"publish_date":"2020-05-11","publish_status":"0","recid":"1468","relation_version_is_last":true,"title":["Low-energy in-line electron holographic imaging of vitreous ice-embedded small biomolecules using a modified scanning electron microscope"],"weko_creator_id":"29","weko_shared_id":29},"updated":"2023-06-26T11:49:52.230519+00:00"}