{"created":"2023-06-26T11:00:53.201180+00:00","id":1479,"links":{},"metadata":{"_buckets":{"deposit":"00967094-9990-4891-b9bd-fd7d0814fcea"},"_deposit":{"created_by":28,"id":"1479","owners":[28],"pid":{"revision_id":0,"type":"depid","value":"1479"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001479","sets":["6:54"]},"author_link":["8947","8946","8945"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-01-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"2302","bibliographicPageStart":"2295","bibliographicVolumeNumber":"5","bibliographic_titles":[{},{"bibliographic_title":"ACS Omega","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ripa, Rosa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shen, Amy Q."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Funari, Riccardo"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Bacterial biofilms are responsible for persistent infections and biofouling, raising serious concerns in both medical and industrial processes. These motivations underpin the need to develop methodologies to study the complex biological structures of biofilms and prevent their formation on medical implants, tools, and industrial apparatuses. Here, we report the detailed comparison of Escherichia coli biofilm development stages (adhesion, maturation, and dispersion) on gold and titanium surfaces by monitoring the changes in both frequency and dissipation of a quartz crystal microbalance (QCM) device, a cheap and reliable microgravimetric sensor which allows the real-time and label-free characterization of various stages of biofilm development. Although gold is the most common electrode material used for QCM sensors, the titanium electrode is also readily available for QCM sensors; thus, QCM sensors with different metal electrodes serve as a simple platform to probe how pathogens interact with different metal substrates. The QCM outcomes are further confirmed by atomic force microscopy and crystal violet staining, thus validating the effectiveness of this surface sensitive sensor for microbial biofilm research. Moreover, because QCM technology can easily modify the substrate types and coatings, QCM sensors also provide well-controlled experimental conditions to study antimicrobial surface treatments and eradication procedures, even on mature biofilms.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"American Chemical Society"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/32064391","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1021/acsomega.9b03540","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://pubs.acs.org/page/policy/authorchoice_termsofuse.html"}]}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubs.acs.org/doi/full/10.1021/acsomega.9b03540","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2020 American Chemical Society"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2470-1343","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-12"}],"displaytype":"detail","filename":"Ripa-2020-Detecting Escherichia coli Biofilm D.pdf","filesize":[{"value":"6.3 MB"}],"format":"application/pdf","license_note":"ACS AuthorChoice\n(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Ripa-2020-Detecting Escherichia coli Biofilm D","url":"https://oist.repo.nii.ac.jp/record/1479/files/Ripa-2020-Detecting Escherichia coli Biofilm D.pdf"},"version_id":"1c514dab-2032-44dc-bc7d-b65dd008641f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Detecting Escherichia coli Biofilm Development Stages on Gold and Titanium by Quartz Crystal Microbalance","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detecting Escherichia coli Biofilm Development Stages on Gold and Titanium by Quartz Crystal Microbalance","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"28","path":["54"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-05-12"},"publish_date":"2020-05-12","publish_status":"0","recid":"1479","relation_version_is_last":true,"title":["Detecting Escherichia coli Biofilm Development Stages on Gold and Titanium by Quartz Crystal Microbalance"],"weko_creator_id":"28","weko_shared_id":28},"updated":"2023-06-26T11:49:39.196626+00:00"}