{"created":"2023-06-26T11:01:03.407223+00:00","id":1672,"links":{},"metadata":{"_buckets":{"deposit":"1706099a-2ff9-4708-9925-4cd561d3d949"},"_deposit":{"created_by":30,"id":"1672","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"1672"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001672","sets":["6:43"]},"author_link":["10031","10030","10028","10032","10029"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"9914","bibliographicVolumeNumber":"10","bibliographic_titles":[{},{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tan, Ee Suan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Izumi, Ryotaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takeuchi, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Isomura, Naoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takemura, Akihiro"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"This study aimed to elucidate the physiological processes of oogenesis in Acropora tenuis. Genes/proteins related to oogenesis were investigated: Vasa, a germ cell marker, vitellogenin (VG), a major yolk protein precursor, and its receptor (LDLR). Coral branches were collected monthly from coral reefs around Sesoko Island (Okinawa, Japan) for histological observation by in situ hybridisation (ISH) of the Vasa (AtVasa) and Low Density Lipoprotein Receptor (AtLDLR) genes and immunohistochemistry (IHC) of AtVasa and AtVG. AtVasa immunoreactivity was detected in germline cells and ooplasm, whereas AtVG immunoreactivity was detected in ooplasm and putative ovarian tissues. AtVasa was localised in germline cells located in the retractor muscles of the mesentery, whereas AtLDLR was localised in the putative ovarian and mesentery tissues. AtLDLR was detected in coral tissues during the vitellogenic phase, whereas AtVG immunoreactivity was found in primary oocytes. Germline cells expressing AtVasa are present throughout the year. In conclusion, Vasa has physiological and molecular roles throughout the oogenic cycle, as it determines gonadal germline cells and ensures normal oocyte development, whereas the roles of VG and LDLR are limited to the vitellogenic stages because they act in coordination with lipoprotein transport, vitellogenin synthesis, and yolk incorporation into oocytes.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Nature Research "}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/32555307","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41598-020-66020-x","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41598-020-66020-x","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2020 The Author(s)"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-08-12"}],"displaytype":"detail","filename":"Tan-2020-Molecular approaches underlying the o.pdf","filesize":[{"value":"6.9 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Tan-2020-Molecular approaches underlying the o","url":"https://oist.repo.nii.ac.jp/record/1672/files/Tan-2020-Molecular approaches underlying the o.pdf"},"version_id":"631b39b9-f995-4719-a253-e4abe4463cd7"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Molecular approaches underlying the oogenic cycle of the scleractinian coral, Acropora tenuis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular approaches underlying the oogenic cycle of the scleractinian coral, Acropora tenuis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["43"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-08-12"},"publish_date":"2020-08-12","publish_status":"0","recid":"1672","relation_version_is_last":true,"title":["Molecular approaches underlying the oogenic cycle of the scleractinian coral, Acropora tenuis"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-06-26T11:45:19.067172+00:00"}