{"created":"2023-06-26T11:01:05.182524+00:00","id":1700,"links":{},"metadata":{"_buckets":{"deposit":"7c2b4863-236c-44dd-b631-7c0c1f271912"},"_deposit":{"created_by":29,"id":"1700","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"1700"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001700","sets":["6:191"]},"author_link":["10234","10231","10235","10232","10227","10237","10228","10229","10230","10236","10233"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06-23","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"120198","bibliographicVolumeNumber":"256","bibliographic_titles":[{},{"bibliographic_title":"Biomaterials","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mohamed, Mahmoud Shaaban"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hazawa, Masaharu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Guillaud, Laurent"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe-Nakayama, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakayama, Mizuho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Hanbo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kodera, Noriyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oshima, Masanobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ando, Toshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wong, Richard W."}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Nuclear pore complex (NPC) is a gating nanomachine with a central selective barrier composed mainly of Nups, which contain intrinsically disordered (non-structured) regions (IDRs) with phenylalanine-glycine (FG) motifs (FG-NUPs). The NPC central FG network dynamics is poorly understood, as FG-NUPs liquid-liquid phase separation (LLPS) have evaded structural characterization. Moreover, the working mechanism of single FG-NUP-biofilaments residing at the central lumen is unknown. In general, flexible biofilaments are expected to be tangled and knotted during their motion and interaction. However, filament knotting visualization in real-time and space has yet to be visualized at the nanoscale. Here, we report a spatiotemporally tracking method for FG-NUP organization with nanoscale resolution, unveiling FG-NUP conformation in NPCs of colorectal cells and organoids at timescales of ~150 ms using high-speed atomic force microscopy (HS-AFM). Tracking of FG-NUP single filaments revealed that single filaments have a heterogeneous thickness in normal and cancer models which in turn affected the filament rotation and motion. Notably, FG-NUPs are overexpressed in various cancers. Using the FG-NUP inhibitor, trans-1,2-cyclohexanediol, we found that central plug size was significantly reduced and incompletely reversible back to filamentous structures in aggressive colon cancer cells and organoids. These data showed a model of FG-NUPs reversible self-assembly devolving into the central plug partial biogenesis. Taken together, HS-AFM enabled the tracking and manipulation of single filaments of native FG-NUPs which has remained evasive for decades.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/32622019","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.biomaterials.2020.120198","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S0142961220304440","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2020 The Authors."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0142-9612","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-09-24"}],"displaytype":"detail","filename":"1-s2.0-S0142961220304440-main.pdf","filesize":[{"value":"3.4 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1-s2.0-S0142961220304440-main","url":"https://oist.repo.nii.ac.jp/record/1700/files/1-s2.0-S0142961220304440-main.pdf"},"version_id":"4c415e5d-250b-48e8-af68-693238dd0edd"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Spatiotemporally tracking of nano-biofilaments inside the nuclear pore complex core","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Spatiotemporally tracking of nano-biofilaments inside the nuclear pore complex core","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"29","path":["191"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-09-24"},"publish_date":"2020-09-24","publish_status":"0","recid":"1700","relation_version_is_last":true,"title":["Spatiotemporally tracking of nano-biofilaments inside the nuclear pore complex core"],"weko_creator_id":"29","weko_shared_id":29},"updated":"2023-06-26T11:44:44.904250+00:00"}