@article{oai:oist.repo.nii.ac.jp:00001750,
 author = {Bourguignon, Thomas and Kinjo, Yukihiro and Villa-Martín, Paula and Coleman, Nicholas V. and Tang, Qian and Arab, Daej A. and Wang, Zongqing and Tokuda, Gaku and Hongoh, Yuichi and Ohkuma, Moriya and Ho, Simon Y.W. and Pigolotti, Simone and Lo, Nathan},
 issue = {19},
 journal = {Current Biology},
 month = {Aug},
 note = {The evolutionary processes that drive variation in genome size across the tree of life remain unresolved. Effective population size (Ne) is thought to play an important role in shaping genome size [1, 2, 3]—a key example being the reduced genomes of insect endosymbionts, which undergo population bottlenecks during transmission [4]. However, the existence of reduced genomes in marine and terrestrial prokaryote species with large Ne indicate that genome reduction is influenced by multiple processes [3]. One candidate process is enhanced mutation rate, which can increase adaptive capacity but can also promote gene loss. To investigate evolutionary forces associated with prokaryotic genome reduction, we performed molecular evolutionary and phylogenomic analyses of nine lineages from five bacterial and archaeal phyla. We found that gene-loss rate strongly correlated with synonymous substitution rate (a proxy for mutation rate) in seven of the nine lineages. However, gene-loss rate showed weak or no correlation with the ratio of nonsynonymous/synonymous substitution rate (dN/dS). These results indicate that genome reduction is largely associated with increased mutation rate, while the association between gene loss and changes in Ne is less well defined. Lineages with relatively high dS and dN, as well as smaller genomes, lacked multiple DNA repair genes, providing a proximate cause for increased mutation rates. Our findings suggest that similar mechanisms drive genome reduction in both intracellular and free-living prokaryotes, with implications for developing a comprehensive theory of prokaryote genome size evolution.},
 pages = {3848--3855.e4},
 title = {Increased Mutation Rate Is Linked to Genome Reduction in Prokaryotes},
 volume = {30},
 year = {2020}
}