{"created":"2023-06-26T11:01:08.051763+00:00","id":1753,"links":{},"metadata":{"_buckets":{"deposit":"cf301d96-2daa-4a2d-9d37-9e57759ae77b"},"_deposit":{"created_by":28,"id":"1753","owners":[28],"pid":{"revision_id":0,"type":"depid","value":"1753"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001753","sets":["6:58","6:74"]},"author_link":["10525","10527","10526","10530","10533","10528","10534","10529","10531","10532","10524"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-07-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"112419","bibliographicVolumeNumber":"165","bibliographic_titles":[{},{"bibliographic_title":"Biosensors and Bioelectronics","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Danielson, Eric"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dindo, Mirco"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Porkovich, Alexander J."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kumar, Pawan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Zhenwei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jain, Prashant"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mete, Trimbak"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ziadi, Zakaria"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kikkeri, Raghavendra"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Laurino, Paola"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sowwan, Mukhles"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Field-effect transistor (FET) biosensors based on low-dimensional materials are capable of highly sensitive and specific label-free detection of various analytes. In this work, a FET biosensor based on graphene decorated with gold nanoparticles (Au NPs) was fabricated for lactose detection in a liquid-gate measurement configuration. This graphene device is functionalized with a carbohydrate recognition domain (CRD) of the human galectin-3 (hGal-3) protein to detect the presence of lactose from the donor effect of lectin – glycan affinity binding on the graphene. Although the detection of lactose is important because of its ubiquitous presence in food and for disease related applications (lactose intolerance condition), in this work we exploit the lectin/carbohydrate interaction to develop a device that in principle could specifically detect very low concentrations of any carbohydrate. The biosensor achieved an effective response to lactose concentrations over a dynamic range from 1 fM to 1 pM (10−15 to 10−12 mol L−1) with a detection limit of 200 aM, a significant enhancement over previous electrochemical graphene devices. The FET sensor response is also specific to lactose at aM concentrations, indicating the potential of a combined lectin and graphene FET (G-FET) sensor to detect carbohydrates at high sensitivity and specificity for disease diagnosis.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/32729537","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.bios.2020.112419","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S0956566320304139","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"This article/chapter was published in Biosensors and Bioelectronics, Volume 165, Eric Danielson, Mirco Dindo, Alexander J. Porkovich, Pawan Kumar, Zhenwei Wang, Prashant Jain, Trimbak Mete, Zakaria Ziadi, Raghavendra Kikkeri, Paola Laurino, Mukhles Sowwan, Non-enzymatic and highly sensitive lactose detection utilizing graphene field-effect transistors, Page 112419, © 2020 Elsevier B.V."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0956-5663","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-03"}],"displaytype":"detail","filename":"B&B Danielson et al for OISTIR - Manuscript.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"B&B Danielson et al for OISTIR - Manuscript","url":"https://oist.repo.nii.ac.jp/record/1753/files/B&B Danielson et al for OISTIR - Manuscript.pdf"},"version_id":"7e7c5c7f-6606-4840-b0a6-cba1779fd2a2"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-03"}],"displaytype":"detail","filename":"B&B Danielson et al for OISTIR - SI.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"B&B Danielson et al for OISTIR - SI","url":"https://oist.repo.nii.ac.jp/record/1753/files/B&B Danielson et al for OISTIR - SI.pdf"},"version_id":"71ec4e12-4aee-4e38-a4c5-bb55530552c1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Lactose biosensor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Field effect transistor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lectin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Graphene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gold nanoparticles","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Non-enzymatic and highly sensitive lactose detection utilizing graphene field-effect transistors","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Non-enzymatic and highly sensitive lactose detection utilizing graphene field-effect transistors","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"28","path":["58","74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-10-30"},"publish_date":"2020-10-30","publish_status":"0","recid":"1753","relation_version_is_last":true,"title":["Non-enzymatic and highly sensitive lactose detection utilizing graphene field-effect transistors"],"weko_creator_id":"28","weko_shared_id":28},"updated":"2023-06-26T11:40:41.576398+00:00"}