{"created":"2023-06-26T11:01:09.892284+00:00","id":1793,"links":{},"metadata":{"_buckets":{"deposit":"dfdc3d80-f26a-43e4-ad9f-dfd5a05a8548"},"_deposit":{"created_by":30,"id":"1793","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"1793"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001793","sets":["6:89"]},"author_link":["10752","10755","10749","10751","10750","10754","10756","10753"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-08-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"35","bibliographicPageStart":"eaba4996","bibliographicVolumeNumber":"6","bibliographic_titles":[{},{"bibliographic_title":"Science Advances","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Lee, Hyuk-Joon"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jeong, Hyeongseop"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hyun, Jaekyung"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ryu, Bumhan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Park, Kunwoong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lim, Hyun-Ho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoo, Jejoong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Woo, Jae-Sung"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Connexin family proteins assemble into hexameric channels called hemichannels/connexons, which function as transmembrane channels or dock together to form gap junction intercellular channels (GJIChs). We determined the cryo-electron microscopy structures of human connexin 31.3 (Cx31.3)/GJC3 hemichannels in the presence and absence of calcium ions and with a hearing-loss mutation R15G at 2.3-, 2.5-, and 2.6-A resolutions, respectively. Compared with available structures of GJICh in open conformation, Cx31.3 hemichannel shows substantial structural changes of highly conserved regions in the connexin family, including opening of calcium ion-binding tunnels, reorganization of salt-bridge networks, exposure of lipid-binding sites, and collocation of amino-terminal helices at the cytoplasmic entrance. We also found that the hemichannel has a pore with a diameter of ~8 A and selectively transports chloride ions. Our study provides structural insights into the permeant selectivity of Cx31.3 hemichannel.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"American Association for the Advancement of Science"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/32923625","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1126/sciadv.aba4996","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://advances.sciencemag.org/content/6/35/eaba4996","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2020 The Author(s), some rights reserved; exclusive licensee American Association for the Advancement of Science. "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2375-2548","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-17"}],"displaytype":"detail","filename":"Lee-2020-Cryo-EM structure of human Cx31.3_GJC.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Lee-2020-Cryo-EM structure of human Cx31.3_GJC","url":"https://oist.repo.nii.ac.jp/record/1793/files/Lee-2020-Cryo-EM structure of human Cx31.3_GJC.pdf"},"version_id":"db8bbc12-996b-4f84-9c3b-b7262e3db834"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["89"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-17"},"publish_date":"2020-11-17","publish_status":"0","recid":"1793","relation_version_is_last":true,"title":["Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-06-26T11:42:46.279081+00:00"}