@article{oai:oist.repo.nii.ac.jp:00001820, author = {Wang, Kai and Tomura, Ryo and Chen, Wei and Kiyooka, Miho and Ishizaki, Hinako and Aizu, Tomoyuki and Minakuchi, Yohei and Seki, Masahide and Suzuki, Yutaka and Omotezako, Tatsuya and Suyama, Ritsuko and Masunaga, Aki and Plessy, Charles and Luscombe, Nicholas M. and Dantec, Christelle and Lemaire, Patrick and Itoh, Takehiko and Toyoda, Atsushi and Nishida, Hiroki and Onuma, Takeshi A.}, issue = {6}, journal = {Development, Growth & Differentiation}, month = {Aug}, note = {The larvacean Oikopleura dioica is a planktonic chordate, and is tunicate that belongs to the closest relatives to vertebrates. Its simple and transparent body, invariant embryonic cell lineages, and short life cycle of five days make it a promising model organism for developmental biology research. The genome browser OikoBase was established in 2013 using Norwegian O. dioica. However, genome information for other populations is not available, even though many researchers have studied local populations. In the present study, we sequenced using Illumina and PacBio RSII technologies the genome of O. dioica from a southwestern Japanese population that was cultured in our laboratory for three years. The genome of Japanese O. dioica was assembled into 576 scaffold sequences with a total length and N50 length of 56.6 Mb and 1.5 Mb, respectively. A total of 18,743 gene models (transcript models) were predicted in the genome assembly, named as OSKA2016. In addition, 19,277 non-redundant transcripts were assembled using RNA-seq data. The OSKA2016 has global sequence similarity of only 86.5% when compared with the OikoBase, highlighting the sequence difference between the two far distant O. dioica populations on the globe. The genome assembly, transcript assembly, and transcript models were incorporated into ANISEED (https://www.aniseed.cnrs.fr/) for genome browsing and blast searches. Moreover, screening of the male-specific scaffolds revealed that over 2.6 Mb of sequence were included in the male-specific Yregion. The genome and transcriptome resources from two distinct populations will be useful datasets for developmental biology, evolutionary biology, and molecular ecology using this model organism.}, pages = {450--461}, title = {A genome database for a Japanese population of the larvacean Oikopleura dioica}, volume = {62}, year = {2020} }