{"created":"2023-06-26T11:01:11.256353+00:00","id":1822,"links":{},"metadata":{"_buckets":{"deposit":"978e1573-f83c-4d03-b7eb-4b3389091faf"},"_deposit":{"created_by":22,"id":"1822","owners":[22],"pid":{"revision_id":0,"type":"depid","value":"1822"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001822","sets":["7:200"]},"author_link":[],"item_10006_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zhang, Shijin","creatorNameLang":"en"}]}]},"item_10006_date_granted_11":{"attribute_name":"Degree Conferral Date","attribute_value_mlt":[{"subitem_dategranted":"2020-11-30"}]},"item_10006_degree_grantor_9":{"attribute_name":"Degree Conferrral Institution","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Okinawa Institute of Science and Technology Graduate University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"38005","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"Degree","attribute_value_mlt":[{"subitem_degreename":"Doctor of Philosophy"}]},"item_10006_description_7":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Molecular self-assembly is a spontaneous process of molecular association through noncovalent bond interaction. Imitating molecular self-assembly and creating novel functional molecular assemblies through synthetic chemistry are important issues in nanotechnology development. Small peptidic molecules are ideal candidates for imitating molecular selfassembly in biological system because of their biocompatibility and easy functionalization for bio-related applications including drug delivery, anti-cancer treatment and biosensors. In the first part (Chapter I), we address the essential role of nanotechnology in daily life, and a brief summarization on nanomaterial species, interaction mode, representative examples from biological system, available characterization methods, classes of artificial nanomaterials and their bio-related applications are outlined. In the second part (Chapter III), we have fabricated well-orientated nanofibrous structure through conjugation of easily deprotonated functional group to self-assembly building block. Via pH adjustment to manipulate molecular charge status, and further mechanical force guiding orientation of the nanofibrous structure. Large scale nanomaterial fabrication is achieved through evaporation casting capillary confinement. In the third part (Chapter IV), higher-order nanomaterial is approached by combination of co-assembly and self-sorting in multiple components. Stimulus such as light is applied on pre-stabilized template to obtain unconventional nanostructures. Two self-sorting components interact with each other at a nanoscale via both ALP and light regulation to reach a higher-order hierarchical assembly. Through ALP and CES - catalysed molecular self-assembly, two self-sorting components interact with each other to deliver a more robust nano-scaffold with potential in cell culture. In the fourth part (Chapter V), precisely subcellular organelle molecular self-assembly is realized by incorporation of the enzyme responsive motif with self-assembly building block. Introducing CES responsive motif to self-assembly peptide enhance cellular uptake of the self-assembly building block, intracellular recovery of the self-assembly peptide enriches on endoplasmic reticulum and thus leading to cancer cell apoptosis. Dual-subcellular-targeting anticancer therapy is reached by conjugating endoplasmic reticulum (ER)-targeted coumarin-functionalized short peptides and DNA alkylating agent (chlorambucil). After being uptaken and hydrolysis by intracellular CES, the coumarin-functionalized peptides self-assemble on ER to introduce ER stress while the chlorambucil targeting the nucleus to show synergistic effect on cancer cells.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10006_dissertation_number_12":{"attribute_name":"Degree Referral Number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第63号"}]},"item_10006_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15102/1394.00001647","subitem_identifier_reg_type":"JaLC"}]},"item_10006_rights_13":{"attribute_name":"Copyright Information","attribute_value_mlt":[{"subitem_rights":"© 2020 The Author."}]},"item_10006_text_24":{"attribute_name":"Exam Date","attribute_value_mlt":[{"subitem_text_value":"2020-09-28"}]},"item_10006_version_type_18":{"attribute_name":"Version Format","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-01"}],"displaytype":"detail","filename":"301.pdf","filesize":[{"value":"10.7 MB"}],"format":"application/pdf","licensefree":"Creative Commons Attribution-NonCommercial 4.0 International(https://creativecommons.org/licenses/by-nc/4.0/) ","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Full_Text","objectType":"fulltext","url":"https://oist.repo.nii.ac.jp/record/1822/files/301.pdf"},"version_id":"d07cb284-089e-4476-9165-1ba9c72a0c67"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-02"}],"displaytype":"detail","filename":"doc.pdf","filesize":[{"value":"42.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Final_Exam_Abstract","objectType":"abstract","url":"https://oist.repo.nii.ac.jp/record/1822/files/doc.pdf"},"version_id":"e5a6f069-e430-4285-91cb-c76182bd0182"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"自己組織化ペプチドの分子設計戦略と生物医療応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"自己組織化ペプチドの分子設計戦略と生物医療応用","subitem_title_language":"ja"},{"subitem_title":"Design and Synthesize Small Molecular Self-Assembling Peptides for Biomedical Applications","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"22","path":["200"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-12-02"},"publish_date":"2020-12-02","publish_status":"0","recid":"1822","relation_version_is_last":true,"title":["自己組織化ペプチドの分子設計戦略と生物医療応用"],"weko_creator_id":"22","weko_shared_id":-1},"updated":"2023-08-31T02:52:51.375029+00:00"}