@article{oai:oist.repo.nii.ac.jp:00001897,
 author = {Koyama-Honda, Ikuko and Fujiwara, Takahiro K. and Kasai, Rinshi S. and Suzuki, Kenichi G.N. and Kajikawa, Eriko and Tsuboi, Hisae and Tsunoyama, Taka A. and Kusumi, Akihiro},
 issue = {12},
 journal = {Journal of Cell Biology},
 month = {Dec},
 note = {Using single-molecule imaging with enhanced time resolutions down to 5 ms, we found that CD59 cluster rafts and GM1 cluster rafts were stably induced in the outer leaflet of the plasma membrane (PM), which triggered the activation of Lyn, H-Ras, and ERK and continually recruited Lyn and H-Ras right beneath them in the inner leaflet with dwell lifetimes <0.1 s. The detection was possible due to the enhanced time resolutions employed here. The recruitment depended on the PM cholesterol and saturated alkyl chains of Lyn and H-Ras, whereas it was blocked by the nonraftophilic transmembrane protein moiety and unsaturated alkyl chains linked to the inner-leaflet molecules. Because GM1 cluster rafts recruited Lyn and H-Ras as efficiently as CD59 cluster rafts, and because the protein moieties of Lyn and H-Ras were not required for the recruitment, we conclude that the transbilayer raft phases induced by the outer-leaflet stabilized rafts recruit lipid-anchored signaling molecules by lateral raft-lipid interactions and thus serve as a key signal transduction platform.},
 title = {High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases},
 volume = {219},
 year = {2020}
}