@phdthesis{oai:oist.repo.nii.ac.jp:00000191,
 author = {スタージンスキ, キャロリン and Starzynski, Caroline},
 month = {2017-12-22, 2018-03-14},
 note = {Since all living organism rely on assimilating environmental nitrogen (N) to promote cell divisions, an efficient system to deal with N scarcity is deterministic for survival. For example, the fission yeast Schizosaccharomyces pombe (S. pombe) withstands long-term N starvation by induction of growth arrest and quiescence entry (G0 phase). In past studies 89 S. pombe genes were found to be required for survival upon G0 phase. Because these are involved in diverse intracellular functions, a clear mechanism for quiescence was difficult to assign. Therefore, we developed a BLAST-based approach to generate phylogenetic profiles and characterize evolutionary conserved metabolic reactions by comparative analysis using prokaryotic databases of Escherichia coli and Bacillus subtilis. We report fourteen proteins fulfilling this study’s homology criteria, half of which (7/14) are localized to the mitochondria. Subsequent in silico analysis suggests the involvement of two functional response mechanisms which comprise oxygen-consuming and glutamate-metabolic reactions. Among these, ∆sod2 showed abnormally low oxygen consumption upon quiescence and loss of regenerative capability. We report that the conserved mechanisms to survive N starvation comprise enzymes which are associated with the regulation of oxygen and glutamateoxoglutarate metabolism.},
 school = {Okinawa Institute of Science and Technology Graduate University},
 title = {分裂酵母S. pombeにおけるG0静止期生存能に寄与する古代代謝機構の発見},
 year = {}
}