{"created":"2023-06-26T11:01:17.851263+00:00","id":1971,"links":{},"metadata":{"_buckets":{"deposit":"2742fef1-6f3c-4009-92c5-ecf517456621"},"_deposit":{"created_by":27,"id":"1971","owners":[27],"pid":{"revision_id":0,"type":"depid","value":"1971"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00001971","sets":["6:213"]},"author_link":["10139","6955","6963","12929","359","6957","9133","6965","10141"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-12-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"4371","bibliographicPageStart":"4357","bibliographicVolumeNumber":"10","bibliographic_titles":[{},{"bibliographic_title":"American Journal of Cancer Research","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kim, Eun Ho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kim, Jeong Yub"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kim, Mi-Sook"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Vares, Guillaume"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohno, Tatsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Akihisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uzawa, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seo, Seung-Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sai, Sei"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Carbon ion radiotherapy (CIRT) is more effective than conventional photon beam radiotherapy in treating osteosarcoma (OSA); however, the outcomes of CIRT alone are still unsatisfactory. In this study, we aimed to investigate whether miR-29b acts as a radiosensitizer for CIRT. The OSA cell lines U2OS and KHOS were treated with carbon ion beam alone, gamma-ray irradiation alone, or in combination with an miR-29b mimic. OSA cell death as well as invasive and migratory abilities were analyzed through viability, colony formation, Transwell, and apoptosis assays. miR-29 expression was downregulated in OSA tissues compared to that in normal tissues and was associated with metastasis and relapse in patients with OSA. Further, miR-29b was found to directly target the transcription factor Sp1 and suppress the activation of the phosphatase and tensin homolog (PTEN)-AKT pathway. Conversely, Sp1 was found to attenuate the inhibitory effects of miR-29b in OSA cells. When used in combination with miR-29b mimic, carbon ion beam markedly inhibited invasion, migration, and proliferation of OSA cells and promoted apoptosis by inhibiting AKT phosphorylation in a Sp1/PTEN-mediated manner. Taken together, miR-29b mimic improved the radiosensitivity of OSA cells via the PTEN-AKT-Sp1 signaling pathway, presenting a novel strategy for the development of carbon ion beam combination therapy.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"e-Century Publishing"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":" info:pmid/33415004","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ajcr.us/AJCR_V10N12.html","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2020 e-Century Publishing"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2156-6976","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-03-09"}],"displaytype":"detail","filename":"ajcr0119320.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/deed.en) ","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ajcr0119320","url":"https://oist.repo.nii.ac.jp/record/1971/files/ajcr0119320.pdf"},"version_id":"8e150081-d6b9-4d8a-bc54-4cd2987ccfd6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"miR-29b","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"carbon ion","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"radiosensitivity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteosarcoma cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"AKT","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PTEN","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sp1","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Molecular mechanisms underlying the enhancement of carbon ion beam radiosensitivity of osteosarcoma cells by miR-29b","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular mechanisms underlying the enhancement of carbon ion beam radiosensitivity of osteosarcoma cells by miR-29b","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"27","path":["213"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-03-09"},"publish_date":"2021-03-09","publish_status":"0","recid":"1971","relation_version_is_last":true,"title":["Molecular mechanisms underlying the enhancement of carbon ion beam radiosensitivity of osteosarcoma cells by miR-29b"],"weko_creator_id":"27","weko_shared_id":27},"updated":"2023-06-26T11:37:53.249014+00:00"}