{"created":"2023-11-27T07:14:30.780515+00:00","id":2000263,"links":{},"metadata":{"_buckets":{"deposit":"d4300b02-2579-4b8b-aff2-b7994b4781c8"},"_deposit":{"created_by":34,"id":"2000263","owner":"34","owners":[34],"pid":{"revision_id":0,"type":"depid","value":"2000263"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:02000263","sets":["6:57"]},"author_link":[],"control_number":"2000263","item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-04-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"163","bibliographicPageStart":"153","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"Current Genetics","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Westlund Emma","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Bergenstråle Axel","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Pokhrel Alaska","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Chan Helena","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Skoglund Ulf","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Daley Daniel O.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Söderström Bill","creatorNameLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Understanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (ALFA), and a corresponding nanobody fused to mEos3.2. The methodology presented is applicable to other bacterial proteins.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"37022498","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00294-023-01266-2","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://creativecommons.org/licenses/by/4.0/)"}]}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://link.springer.com/article/10.1007/s00294-023-01266-2","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2023 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0172-8083","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"1432-0983","subitem_source_identifier_type":"EISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-11-27"}],"filename":"Westlund-Application of nanotags and nanobodie.pdf","filesize":[{"value":"10.7 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://oist.repo.nii.ac.jp/record/2000263/files/Westlund-Application of nanotags and nanobodie.pdf"},"version_id":"9ced6cc6-5394-4e6f-ac67-c5eb9fdefa9d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ALFA-tag|Nanotags|E. coli|FtsZ|STED|PALM","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"34","path":["57"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-11-27"},"publish_date":"2023-11-27","publish_status":"0","recid":"2000263","relation_version_is_last":true,"title":["Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli"],"weko_creator_id":"34","weko_shared_id":-1},"updated":"2024-01-15T02:33:07.290788+00:00"}