{"created":"2024-05-22T03:36:06.893497+00:00","id":2000496,"links":{},"metadata":{"_buckets":{"deposit":"3f774d90-490b-4450-a7d7-58d32c6735cc"},"_deposit":{"created_by":33,"id":"2000496","owner":"33","owners":[33],"pid":{"revision_id":0,"type":"depid","value":"2000496"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:02000496","sets":["7:1707111347047"]},"author_link":[],"control_number":"2000496","item_10006_creator_3":{"attribute_name":"著者(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Moore, Billy","creatorNameLang":"en"}]}]},"item_10006_date_granted_11":{"attribute_name":"Degree Conferral Date","attribute_value_mlt":[{"subitem_dategranted":"2024-03-31"}]},"item_10006_degree_grantor_9":{"attribute_name":"Degree Conferrral Institution","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Okinawa Institute of Science and Technology Graduate University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"38005","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"Degree","attribute_value_mlt":[{"subitem_degreename":"Doctor of Philosophy"}]},"item_10006_description_7":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Coral reef fish are currently under pressure from anthropogenic CO2 emissions which are exacerbating the greenhouse effect and driving long-term changes in mean sea surface temperatures, whilst also increasing the frequency and intensity of marine heatwaves. Increasing temperatures threaten to push coral reef fish beyond their natural thermal limits, with previous studies indicating this will elicit a range of adverse physiological and molecular responses. Nevertheless, the immediate, acclimatory and legacy effects of increasing temperature on early life-stage coral reef fish are largely unclear, with the molecular mechanisms underpinning many of the previously observed phenotypes still unidentified. To address these knowledge gaps, I use a combination of genome sequencing, transcriptomics, and physiological measurements to investigate the response of early life-stage clownfish (Amphiprion ocellaris) to temperatures associated with ocean warming and marine heatwaves. Firstly, I present two high quality chromosome-scale genomes for the anemonefish Amphiprion ocellaris and Amphiprion clarkii, that will aid future studies of these species. Then, via a series of aquaria-based experiments I show that A. ocellaris exhibit a range of physiological changes at +3°C, as larvae develop faster, and both larvae and juvenile clownfish display elevated metabolic rates. However, I also show that early exposure to +3 °C may have an acclamatory effect, as the effect of acute temperature stress on metabolic rates are reduced with increasing developmental exposure. Furthermore, via stage- and tissue-specific transcriptome sequencing of larvae and juveniles I show widespread changes in gene expression at +3 °C. These genes encompass a range of biological functions including immune response, epigenetic reprogramming, neurotransmission, heat-stress, liver fibrosis and insulin signalling. Overall, these results indicate that A. ocellaris may experience a significantly different developmental regime if they hatch and develop at +3 °C, with these changes likely to impact future population persistence. Moreover, the comprehensive transcriptomic sequencing conducted here, advances our understanding of how coral reef fish will respond to future climate change at the molecular level. Chapters one and two of this thesis have been peer-reviewed and published, and chapter three is currently in preparation for journal submission.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"Degree Referral Number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第149号"}]},"item_10006_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15102/0002000496","subitem_identifier_reg_type":"JaLC"}]},"item_10006_rights_13":{"attribute_name":"Copyright Information","attribute_value_mlt":[{"subitem_rights":"© 2024 The Author."}]},"item_10006_text_24":{"attribute_name":"Exam Date","attribute_value_mlt":[{"subitem_text_value":"2024-03-06"}]},"item_10006_version_type_18":{"attribute_name":"Version Format","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2025-04-03"}],"filename":"MooreBillyFulltext.pdf","filesize":[{"value":"4.4 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://oist.repo.nii.ac.jp/record/2000496/files/MooreBillyFulltext.pdf"},"version_id":"c753a248-2f35-4c38-97b8-00c79f20fb3d"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-05-22"}],"filename":"MooreBillyExamAbstract.pdf","filesize":[{"value":"48 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://oist.repo.nii.ac.jp/record/2000496/files/MooreBillyExamAbstract.pdf"},"version_id":"0521a880-b11b-4b23-b943-4f4ea0915379"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-05-22"}],"filename":"MooreBillySummary.pdf","filesize":[{"value":"430 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"summary","url":"https://oist.repo.nii.ac.jp/record/2000496/files/MooreBillySummary.pdf"},"version_id":"a99dc22b-d774-4eeb-90ba-df4b345096f7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fish ecology | Fish physiology | Genomics | Genetics | Climate Change","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"温暖化がカクレクマノミに及ぼす生理的･遺伝的影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"温暖化がカクレクマノミに及ぼす生理的･遺伝的影響","subitem_title_language":"ja"},{"subitem_title":"Physiological and Transcriptomic Response of Early-Life Stage Clownfish to Future Ocean Warming and Marine Heatwaves","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"33","path":["1707111347047"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-05-22"},"publish_date":"2024-05-22","publish_status":"0","recid":"2000496","relation_version_is_last":true,"title":["温暖化がカクレクマノミに及ぼす生理的･遺伝的影響"],"weko_creator_id":"33","weko_shared_id":-1},"updated":"2024-05-28T07:11:43.638953+00:00"}