{"created":"2024-06-04T07:07:36.579165+00:00","id":2000518,"links":{},"metadata":{"_buckets":{"deposit":"1fd4b08d-0c9c-4156-bfa8-55b6a3e1e7b9"},"_deposit":{"created_by":33,"id":"2000518","owner":"33","owners":[33],"pid":{"revision_id":0,"type":"depid","value":"2000518"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:02000518","sets":["7:1707111347047"]},"author_link":[],"control_number":"2000518","item_10006_creator_3":{"attribute_name":"著者(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Desforges, Miles","creatorNameLang":"en"}]}]},"item_10006_date_granted_11":{"attribute_name":"Degree Conferral Date","attribute_value_mlt":[{"subitem_dategranted":"2024-05-31"}]},"item_10006_degree_grantor_9":{"attribute_name":"Degree Conferrral Institution","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Okinawa Institute of Science and Technology Graduate University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"38005","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"Degree","attribute_value_mlt":[{"subitem_degreename":"Doctor of Philosophy"}]},"item_10006_description_7":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"The precise spatiotemporal dynamics of neuromodulator release in the cortex remain elusive. These release patterns determine receptor activation, as receptors with different affinities are activated by different concentrations, and thereby distances, from the release site. While neuromodulator nuclei have been extensively characterised, technological challenges have hindered detailed cortical investigation. This thesis clarifies the release patterns of dopamine, noradrenaline, and serotonin in the secondary motor cortex (M2) using a novel orthogonalized Go/NoGo task developed for head-fixed mice. We dissect neuromodulator activity at the intersection of locomotion and unconditioned stimuli (US). Employing two photon microscopy and novel genetically encoded sensors, we achieved high-resolution imaging unattainable with previous techniques. Our findings indicate that serotonin, noradrenaline, and dopamine are robustly correlated with locomotion transitions, exhibiting significant increases from rest to locomotion and decreases during the reverse. Cross-correlation analysis suggests neuromodulator activity precedes locomotion onset, potentially facilitating motor behaviours. Unexpectedly, both appetitive (sucrose) and aversive (air-puff) stimuli elicited fluorescence decreases in all neuromodulators. Serotonin and noradrenaline showed stronger, more consistent responses during movement than rest, while dopamine responses were more consistent across locomotion states. Detailed analysis of activity maxima and minima within individual trials revealed significant variability and complexity. Contrary to expectations, no neuromodulator responses to US-predicting cues were observed, despite anticipatory behavioural changes. This highlights the need for further investigation into sensory cue processing in M2. Our findings suggest a complex multiplexing of information in M2, whereby neuromodulator activity is more influenced by locomotive state than appetitive or aversive stimuli delivery.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"Degree Referral Number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第153号"}]},"item_10006_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15102/0002000518","subitem_identifier_reg_type":"JaLC"}]},"item_10006_rights_13":{"attribute_name":"Copyright Information","attribute_value_mlt":[{"subitem_rights":"© 2024 The Author."}]},"item_10006_text_24":{"attribute_name":"Exam Date","attribute_value_mlt":[{"subitem_text_value":"2024-02-29"}]},"item_10006_version_type_18":{"attribute_name":"Version Format","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-06-04"}],"filename":"DesforgesMilesFulltext.pdf","filesize":[{"value":"42.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://oist.repo.nii.ac.jp/record/2000518/files/DesforgesMilesFulltext.pdf"},"version_id":"626cb5e1-5fd6-4b8d-a888-0a61aa6aa145"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-06-04"}],"filename":"DesforgesMilesExamAbstract.pdf","filesize":[{"value":"48 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://oist.repo.nii.ac.jp/record/2000518/files/DesforgesMilesExamAbstract.pdf"},"version_id":"6018ca0f-5aa0-42a6-a8b1-6edc038e14a0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Behavioral neuroscience | Neurophysiology, especially neuromodulators | Two-photon imaging | Neuromodulator biosensors","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"マウス運動前野におけるドーパミン、ノルアドレナリン、セロトニンの時空間応答の2光子イメージング","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マウス運動前野におけるドーパミン、ノルアドレナリン、セロトニンの時空間応答の2光子イメージング","subitem_title_language":"ja"},{"subitem_title":"Characterisation of Serotonin, Noradrenaline and Dopamine Release in the Motor Cortex","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"33","path":["1707111347047"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-04"},"publish_date":"2024-06-04","publish_status":"0","recid":"2000518","relation_version_is_last":true,"title":["マウス運動前野におけるドーパミン、ノルアドレナリン、セロトニンの時空間応答の2光子イメージング"],"weko_creator_id":"33","weko_shared_id":-1},"updated":"2024-06-11T04:38:38.902907+00:00"}