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Optimization of Exon-Skipping Riboswitches and Their Applications to Control Mammalian Cell Fate
https://oist.repo.nii.ac.jp/records/2000689
https://oist.repo.nii.ac.jp/records/2000689e5948645-88a5-4fe4-892f-e6ecebc6f1f6
| Name / File | License | Actions |
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Nomura-2024-Optimization of Exon-Skipping Ribo.pdf (3.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| PubDate | 2024-11-15 | |||||||||||||||||
| Title | ||||||||||||||||||
| Title | Optimization of Exon-Skipping Riboswitches and Their Applications to Control Mammalian Cell Fate | |||||||||||||||||
| Language | en | |||||||||||||||||
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| Language | eng | |||||||||||||||||
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| Language | en | |||||||||||||||||
| Subject Scheme | Other | |||||||||||||||||
| Subject | riboswitch | aptamer | alternative splicing | cell-fate control | kill switch | Cells | Differentiation | Fluorescence | Genetics | Peptides and proteins | |||||||||||||||||
| Resource Type | ||||||||||||||||||
| Resource Type Identifier | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| Resource Type | journal article | |||||||||||||||||
| Access Right | ||||||||||||||||||
| Access Rights | open access | |||||||||||||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||
| Author |
Nomura, Yoko
× Nomura, Yoko
× Kim, Narae
× Zhu, Bochen
× Hamzah, Muhammad
× Zhang, Haifeng
× Yokobayashi, Yohei
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| Bibliographic Information |
en : ACS Synthetic Biology Volume Number 13, Issue Number 10, p. 3246-3255, Issue Date 2024-09-25 |
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| Abstract | ||||||||||||||||||
| Description Type | Abstract | |||||||||||||||||
| Description | Mammalian riboswitches that can regulate transgene expression via RNA–small molecule interaction have promising applications in medicine and biotechnology, as they involve no protein factors that can induce immunogenic reactions and are not dependent on specially engineered promoters. However, the lack of cell-permeable and low-toxicity small molecules and cognate aptamers that can be exploited as riboswitches and the modest switching performance of mammalian riboswitches have limited their applications. In this study, we systematically optimized the design of a riboswitch that regulates exon skipping via an RNA aptamer that binds ASP2905. We examined two design strategies to modulate the stability of the aptamer base stem that blocks the 5′ splice site to fine-tune the riboswitch characteristics. Furthermore, an optimized riboswitch was used to generate a mouse embryonic stem cell line that can be chemically induced to differentiate into myogenic cells by activating Myod1 expression and a human embryonic kidney cell line that can be induced to trigger apoptosis by activating BAX expression. The results demonstrate the tight chemical regulation of transgenes in mammalian cells to control their phenotype without exogenous protein factors. | |||||||||||||||||
| Language | en | |||||||||||||||||
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| Publisher | American Chemical Society | |||||||||||||||||
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| Source Identifier Type | EISSN | |||||||||||||||||
| Source Identifier | 2161-5063 | |||||||||||||||||
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| Relation Type | isIdenticalTo | |||||||||||||||||
| Identifier Type | PMID | |||||||||||||||||
| Related Identifier | 39318128 | |||||||||||||||||
| DOI | ||||||||||||||||||
| Relation Type | isIdenticalTo | |||||||||||||||||
| Identifier Type | DOI | |||||||||||||||||
| Related Identifier | 10.1021/acssynbio.4c00295 | |||||||||||||||||
| Rights | ||||||||||||||||||
| Rights | © 2024 The Authors | |||||||||||||||||
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| Identifier Type | URI | |||||||||||||||||
| Related Identifier | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||||||||||||||
| Related Title | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International | |||||||||||||||||
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| Identifier Type | URI | |||||||||||||||||
| Related Identifier | https://pubs.acs.org/doi/10.1021/acssynbio.4c00295 | |||||||||||||||||
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| Version Type | VoR | |||||||||||||||||
| Version Type Resource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
