WEKO3
Item
Epigenetic Regulation of Cold Stress Response and Memory in Arabidopsis thaliana
https://doi.org/10.15102/0002000974
https://doi.org/10.15102/0002000974fe9157d8-e0ae-44c5-a426-2babf9b20f61
| Name / File | License | Actions |
|---|---|---|
SadykovaMunissaFulltext.pdf (27.1 MB)
Download is available from 2026/9/3.
|
.png)
|
|
|
SadykovaMunissaExamAbstract.pdf (48 KB)
|
|
|
|
SadykovaMunissaSummary.pdf (79 KB)
|
|
| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| PubDate | 2025-09-12 | |||||||
| Title | ||||||||
| Title | シロイヌナズナにおける寒冷ストレス応答と記憶のエピジェネティック制御 | |||||||
| Language | ja | |||||||
| Title | ||||||||
| Title | Epigenetic Regulation of Cold Stress Response and Memory in Arabidopsis thaliana | |||||||
| Language | en | |||||||
| Language | ||||||||
| Language | eng | |||||||
| Resource Type | ||||||||
| Resource Type Identifier | http://purl.org/coar/resource_type/c_db06 | |||||||
| Resource Type | doctoral thesis | |||||||
| Identifier Registration | ||||||||
| Identifier Registration | 10.15102/0002000974 | |||||||
| Identifier Registration Type | JaLC | |||||||
| Access Right | ||||||||
| Access Rights | open access | |||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| Author |
Sadykova, Munissa
× Sadykova, Munissa
|
|||||||
| Abstract | ||||||||
| Description Type | Abstract | |||||||
| Description | Plants have developed various strategies to cope with environmental stress, including the ability to “remember” previous stress events, which allows them to produce a more efficient response upon re-exposure – a phenomenon known as stress priming. In Arabidopsis thaliana, cold stress priming is associated with transcriptomic and metabolomic changes that confer increased cold tolerance. While transcriptional responses to cold priming – such as accelerated or amplified gene expression, gene sensitization, and downregulation – are well documented, the epigenetic mechanisms underlying these responses remain poorly understood. In this study, I examined the transcriptome and DNA methylation dynamics in primed and nonprimed A. thaliana under cold stress, using wild-type and DNA methylation-deficient mutants to investigate the epigenetic regulation of cold tolerance and priming. I identified 500 cold memory genes in the wild-type plants which were redistributed in the epigenetic mutants. Cold stress induced widespread DNA methylation changes across all cytosine contexts (CG, CHG, CHH). In addition, cold stress memory genes were significantly hypo-methylated compared to the background genes, suggesting that DNA hypo-methylation can be a hallmark of cold stress memory in A. thaliana. Cold stress memory genes were also enriched for active histone marks, such as H3K4me3 and H3K36me3, and the histone variants H2A.Z and H3.3. The key regulators of cold stress response, including CBF/DREB1 and COR genes, were over-expressed in the met1 DNA methylation mutant, suggesting the role of CG methylation in cold stress response regulation. Finally, I identified cold-responsive and cold-primed non-coding RNAs (ncRNAs) and transposable elements (TEs) that might be involved in cold acclimation and memory. These findings suggest that DNA methylation dynamics contribute to transcriptional memory of cold stress, highlighting the significance of epigenome regulation in stress tolerance and adaptation in plants. |
|||||||
| Language | en | |||||||
| Exam Date | ||||||||
| 2025-07-28 | ||||||||
| Degree Conferral Date | ||||||||
| Date Granted | 2025-08-31 | |||||||
| Degree | ||||||||
| Degree Name | Doctor of Philosophy | |||||||
| Degree Referral Number | ||||||||
| Dissertation Number | 甲第203号 | |||||||
| Degree Conferrral Institution | ||||||||
| Degree Grantor Name Identifier Scheme | kakenhi | |||||||
| Degree Grantor Name Identifier | 38005 | |||||||
| Degree Grantor Name | Okinawa Institute of Science and Technology Graduate University | |||||||
| Version Format | ||||||||
| Version Type | VoR | |||||||
| Version Type Resource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| Copyright Information | ||||||||
| Rights | © 2025 The Author. | |||||||
