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Exploration and Engineering of Protein Sequence Space via Insertions and Deletions
https://doi.org/10.15102/0002000984
https://doi.org/10.15102/0002000984bc90897c-e102-4297-8210-a91663ab1cae
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KozomeDanFulltext.pdf (4.7 MB)
Download is available from 2026/10/4.
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KozomeDanExamAbstract.pdf (48 KB)
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KozomeDanSumarry.pdf (66 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| PubDate | 2025-10-15 | |||||||
| Title | ||||||||
| Title | 挿入と欠失（InDels）を介したタンパク質配列空間の探索と設計 | |||||||
| Language | ja | |||||||
| Title | ||||||||
| Title | Exploration and Engineering of Protein Sequence Space via Insertions and Deletions | |||||||
| Language | en | |||||||
| Language | ||||||||
| Language | eng | |||||||
| Keyword | ||||||||
| Subject Scheme | Other | |||||||
| Subject | biochemistry | biophysics | ancestral sequence reconstruction | molecular evolution | |||||||
| Resource Type | ||||||||
| Resource Type Identifier | http://purl.org/coar/resource_type/c_db06 | |||||||
| Resource Type | doctoral thesis | |||||||
| Identifier Registration | ||||||||
| Identifier Registration | 10.15102/0002000984 | |||||||
| Identifier Registration Type | JaLC | |||||||
| Access Right | ||||||||
| Access Rights | open access | |||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| Author |
神初 弾
× 神初 弾
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| Author |
Kozome, Dan
× Kozome, Dan
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| Abstract | ||||||||
| Description Type | Abstract | |||||||
| Description | Proteins are essential molecules for sustaining life and its diversity, mainly composed of 20 amino acids. Despite the vast theoretical possibilities of protein sequence space, nature utilizes only a small, selectively purified area. While previous studies have mainly focused on exploring sequence space with fixed sequence lengths, investigations involving insertions and deletions (InDels) have been limited due to the immense complexity of the sequence space. This thesis addresses this unexplored frontier, providing a systematic exploration and analysis of protein sequence space. The research begins with a comprehensive investigation of the sequence space in the lysozyme-like fold superfamily, establishing a global map of its sequence space in Chapter 1. Chapter 2 focuses on the GH19 chitinase family - an essential enzyme for degradation of fungal pathogen - a selected unexplored region within this space where functional innovations, mediated by InDels, have occurred in remote region of the protein. Chapter 3 extends the findings by exploring strategies to expand and apply these newly explored regions, revealing their broader potential for understanding evolutionary pathways and engineering novel functionalities. Through this work, the thesis deepens our understanding of the dynamics and diversity of protein sequence space, shedding light on evolutionary innovation and providing a foundation for advancing enzyme design and engineering. | |||||||
| Language | en | |||||||
| Exam Date | ||||||||
| 2025-08-28 | ||||||||
| Degree Conferral Date | ||||||||
| Date Granted | 2025-09-30 | |||||||
| Degree | ||||||||
| Degree Name | Doctor of Philosophy | |||||||
| Degree Referral Number | ||||||||
| Dissertation Number | 甲第208号 | |||||||
| Degree Conferrral Institution | ||||||||
| Degree Grantor Name Identifier Scheme | kakenhi | |||||||
| Degree Grantor Name Identifier | 38005 | |||||||
| Degree Grantor Name | Okinawa Institute of Science and Technology Graduate University | |||||||
| Version Format | ||||||||
| Version Type | VoR | |||||||
| Version Type Resource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| Copyright Information | ||||||||
| Rights | © 2025 The Author. | |||||||
