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CREB Mediated Hypoxia Response in Nematostella vectensis
https://doi.org/10.15102/0002001097
https://doi.org/10.15102/0002001097ee95822b-99be-44e0-ab46-2cc5cb878efa
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QamarShaguftaFulltext.pdf (56.1 MB)
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QamarShaguftaExamAbstract.pdf (64 KB)
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QamarShaguftaSummary.pdf (94 KB)
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| Item type | デフォルトアイテムタイプ（フル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| PubDate | 2026-01-30 | |||||||
| Title | ||||||||
| Title | ネマトステラにおけるCREB依存的低酸素応答 | |||||||
| Language | ja | |||||||
| Title | ||||||||
| Title | CREB Mediated Hypoxia Response in Nematostella vectensis | |||||||
| Language | en | |||||||
| Creator |
Qamar, Shagufta
× Qamar, Shagufta
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| Access Rights | ||||||||
| Access Rights | open access | |||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| Rights | ||||||||
| Rights | © 2025 The Author. | |||||||
| Rights | ||||||||
| Rights Resource | https://creativecommons.org/licenses/by-nc/4.0/ | |||||||
| Rights | Creative Commons Attribution-NonCommercial 4.0 International | |||||||
| Subject | ||||||||
| Subject Scheme | Other | |||||||
| Subject | Cnidaria | transcriptome | possible link between rising oxygen levels and early metazoan evolution | |||||||
| Description | ||||||||
| Description Type | Abstract | |||||||
| Description | Oxygen availability is hypothesised as one of the critical drivers of metazoan evolution and diversification. The earliest metazoans likely emerged in shallow marine shelves in the Neoproterozoic era, a period characterised by a fluctuating and spatially heterogenous redox environment. This would have imposed physiological constraints on the early animals, selecting for oxygen-responsive and stress-adaptive traits. Understanding how ancestral metazoans navigated these dynamic oxygen landscapes is therefore essential for reconstructing the evolutionary backdrop of the emergence of animal life on Earth. To investigate these mechanisms, the cnidarian Nematostella vectensis, a representative of early-diverging metazoans, is used to comprehensively investigate the molecular and developmental responses to hypoxia stress. N. vectensis embryogenesis is demonstrated to be oxygen-dependent, with hypoxia inducing a reversible developmental arrest. Transcriptomic profiling reveals an upregulation of cAMP-response element-binding (CREB) family genes and enrichment of CREB signalling pathways under hypoxia. Targeted knockdown of key CREB homologues, followed by hypoxia culture, reveals that CREB activity is essential for coordinating the embryonic response to low oxygen: CREB activation under hypoxia induces the reversible developmental arrest, whereas loss of CREB allows embryos to aberrantly bypass this arrest, producing irreversible defects in endoderm specification. Transcriptomic profiling of CREB knockdown embryos highlights a core CREB-regulated hypoxia-response network enriched for genes involved in extracellular matrix organization, cell–cell adhesion, and tissue architecture. Comparative analysis shows that this CREB-regulated network is deeply conserved across metazoans and non-metazoan lineages. Together, these findings position CREB as an ancient integrator that couples environmental oxygen availability to morphogenetic events, enhancing developmental robustness during early animal evolution in the fluctuating redox landscapes in Earth’s history. |
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| Language | en | |||||||
| Resource Type | ||||||||
| Resource Type Identifier | http://purl.org/coar/resource_type/c_db06 | |||||||
| Resource Type | doctoral thesis | |||||||
| Version Type | ||||||||
| Version Type | VoR | |||||||
| Version Type Resource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| Identifier Registration | ||||||||
| Identifier Registration | 10.15102/0002001097 | |||||||
| Identifier Registration Type | JaLC | |||||||
| Dissertation Number | ||||||||
| Dissertation Number | 甲第217号 | |||||||
| Degree Name | ||||||||
| Language | en | |||||||
| Degree Name | Doctor of Philosophy | |||||||
| Date Granted | ||||||||
| Date Granted | 2025-11-30 | |||||||
| Degree Grantor | ||||||||
| Degree Grantor Name Identifier Scheme | kakenhi | |||||||
| Degree Grantor Name Identifier | 38005 | |||||||
| Language | en | |||||||
| Degree Grantor Name | Okinawa Institute of Science and Technology Graduate University | |||||||
