@article{oai:oist.repo.nii.ac.jp:00002032,
 author = {Bokelmann, Lukas and Nickel, Olaf and Maricic, Tomislav and Pääbo, Svante and Meyer, Matthias and Borte, Stephan and Riesenberg, Stephan},
 issue = {1},
 journal = {Nature Communications},
 month = {Mar},
 note = {Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.},
 title = {Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP},
 volume = {12},
 year = {2021}
}