{"created":"2023-06-26T11:01:20.502796+00:00","id":2032,"links":{},"metadata":{"_buckets":{"deposit":"ecdcfdc6-343a-4513-947d-8bae222fa46d"},"_deposit":{"created_by":30,"id":"2032","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"2032"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002032","sets":["6:221"]},"author_link":["13194","13193","13192","13190","13195","13191","13196"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-03-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"1467","bibliographicVolumeNumber":"12","bibliographic_titles":[{},{"bibliographic_title":"Nature Communications","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Bokelmann, Lukas"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nickel, Olaf"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maricic, Tomislav"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Pääbo, Svante"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Meyer, Matthias"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Borte, Stephan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Riesenberg, Stephan"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Nature portfolio"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/33674580","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41467-021-21627-0","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41467-021-21627-0","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2041-1723","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-06"}],"displaytype":"detail","filename":"Bokelmann-2021-Point-of-care bulk testing for.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Bokelmann-2021-Point-of-care bulk testing for","url":"https://oist.repo.nii.ac.jp/record/2032/files/Bokelmann-2021-Point-of-care bulk testing for.pdf"},"version_id":"7013ec59-86eb-440d-9dd3-f356f7f4d8c0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["221"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-05-06"},"publish_date":"2021-05-06","publish_status":"0","recid":"2032","relation_version_is_last":true,"title":["Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-07-31T03:43:04.018454+00:00"}