{"created":"2023-06-26T11:01:20.624438+00:00","id":2034,"links":{},"metadata":{"_buckets":{"deposit":"2f6b65a6-2db2-4cd3-9275-fbce01da5e9a"},"_deposit":{"created_by":30,"id":"2034","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"2034"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002034","sets":["6:46"]},"author_link":["13209","13210","13211"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-03-25","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"609287","bibliographicVolumeNumber":"8","bibliographic_titles":[{},{"bibliographic_title":"Frontiers in Marine Science","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chuang, Po-Shun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishikawa, Kota"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mitarai, Satoshi"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Contemporary advances in microfluidic and molecular techniques have enabled coral studies to shift from reef and colony scales to polyp- and molecular-level investigations. Polyp bail-out provides an alternative approach to acquire solitary polyps for studies at finer scales. Although induction of polyp bail-out has been reported in several studies, polyp health after bail-out has not been investigated. In this study, we monitored morphological and genetic changes in Pocillopora acuta polyps after bail-out induced by hyperosmosis. In isosmotic conditions, over 80% of bailed-out polyps survived, of which half regenerated normal polyp morphology within 5 days, including a polarized polyp body, extended tentacles, and a distinguishable oral disk. In contrast, the remaining polyps degenerated into tissue ball-like structures that resemble multicellular aggregates reported in earlier studies. In morphologically recovered polyps, transcriptomic analysis showed that ∼87% of genes altered during bail-out induction recovered from stress status, suggesting resumption of metabolism, cell division, and immunity, while in degenerated polyps, only ∼71% of genes recovered. Quantitative polymerase chain reaction data further demonstrated that genetic recovery of energy production, cell proliferation, and immune response was achieved in morphologically recovered polyps within 3 days after bail-out, but was not fully accomplished in degenerated polyps even after 5 days. Our findings indicate that solitary polyps generated by hyperosmosis-induced bail-out can recover rapidly from physiological stress under laboratory conditions, suggesting that bailed-out polyps could be used as new models for coral research.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Frontiers Media"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.3389/fmars.2021.609287","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.frontiersin.org/articles/10.3389/fmars.2021.609287/full","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2021 Chuang, Ishikawa and Mitarai."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2296-7745","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-06"}],"displaytype":"detail","filename":"Chuang-2021-Morphological and Genetic Recovery.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Chuang-2021-Morphological and Genetic Recovery","url":"https://oist.repo.nii.ac.jp/record/2034/files/Chuang-2021-Morphological and Genetic Recovery.pdf"},"version_id":"caeeaaeb-3824-4ac2-a939-eddd6137abc2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"polyp bail-out","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"micropropagation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"polyp recovery","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"genetics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"metabolism","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Morphological and Genetic Recovery of Coral Polyps After Bail-Out","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Morphological and Genetic Recovery of Coral Polyps After Bail-Out","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["46"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-05-06"},"publish_date":"2021-05-06","publish_status":"0","recid":"2034","relation_version_is_last":true,"title":["Morphological and Genetic Recovery of Coral Polyps After Bail-Out"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-06-26T11:38:45.799757+00:00"}