@article{oai:oist.repo.nii.ac.jp:00002060,
 author = {Georgiou, Leonidas and Echeverría, Anaí and Georgiou, Achilleas and Kuhn, Bernd},
 issue = {6},
 journal = {Science Advances},
 month = {Feb},
 note = {Astrocytes exhibit localized Ca2+ microdomain (MD) activity thought to be actively involved in information processing in the brain. However, functional organization of Ca2+ MDs in space and time in relationship to behavior and neuronal activity is poorly understood. Here, we first show that adeno-associated virus (AAV) particles transfer anterogradely from axons to astrocytes. Then, we use this axoastrocytic AAV transfer to express genetically encoded Ca2+ indicators at high-contrast circuit specifically. In combination with two-photon microscopy and unbiased, event-based analysis, we investigated cortical astrocytes embedded in the vibrissal thalamocortical circuit. We found a wide range of Ca2+ MD signals, some of which were ultrafast (≤300 ms). Frequency and size of signals were extensively increased by locomotion but only subtly with sensory stimulation. The overlay of these signals resulted in behavior-dependent maps with characteristic Ca2+ activity hotspots, maybe representing memory engrams. These functional subdomains are stable over days, suggesting subcellular specialization.},
 title = {Ca2+ activity maps of astrocytes tagged by axoastrocytic AAV transfer},
 volume = {8},
 year = {2022}
}