{"created":"2023-06-26T11:01:22.008085+00:00","id":2060,"links":{},"metadata":{"_buckets":{"deposit":"d3a79528-e72b-44a3-9f96-01d07c7a7e62"},"_deposit":{"created_by":31,"id":"2060","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"2060"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002060","sets":["6:38"]},"author_link":["13352","13350","13349","13351"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-02-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"Science Advances"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Georgiou, Leonidas"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Echeverría, Anaí"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Georgiou, Achilleas"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuhn, Bernd"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Astrocytes exhibit localized Ca2+ microdomain (MD) activity thought to be actively involved in information processing in the brain. However, functional organization of Ca2+ MDs in space and time in relationship to behavior and neuronal activity is poorly understood. Here, we first show that adeno-associated virus (AAV) particles transfer anterogradely from axons to astrocytes. Then, we use this axoastrocytic AAV transfer to express genetically encoded Ca2+ indicators at high-contrast circuit specifically. In combination with two-photon microscopy and unbiased, event-based analysis, we investigated cortical astrocytes embedded in the vibrissal thalamocortical circuit. We found a wide range of Ca2+ MD signals, some of which were ultrafast (≤300 ms). Frequency and size of signals were extensively increased by locomotion but only subtly with sensory stimulation. The overlay of these signals resulted in behavior-dependent maps with characteristic Ca2+ activity hotspots, maybe representing memory engrams. These functional subdomains are stable over days, suggesting subcellular specialization.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"American Association for the Advancement of Science"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/35138891","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1126/sciadv.abe5371","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.science.org/doi/10.1126/sciadv.abe5371","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2022 The Authors"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2375-2548","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-19"}],"displaytype":"detail","filename":"Georgiou-2022-Ca(+) activity maps of astrocyte.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","license_note":"CC BY 4.0\nCreative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Georgiou-2022-Ca(+) activity maps of astrocyte","url":"https://oist.repo.nii.ac.jp/record/2060/files/Georgiou-2022-Ca(+) activity maps of astrocyte.pdf"},"version_id":"bc45ea77-1ba1-40be-a23b-2221ca81d9d8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ca2+ activity maps of astrocytes tagged by axoastrocytic AAV transfer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ca2+ activity maps of astrocytes tagged by axoastrocytic AAV transfer"}]},"item_type_id":"10001","owner":"31","path":["38"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-04-18"},"publish_date":"2022-04-18","publish_status":"0","recid":"2060","relation_version_is_last":true,"title":["Ca2+ activity maps of astrocytes tagged by axoastrocytic AAV transfer"],"weko_creator_id":"31","weko_shared_id":31},"updated":"2023-06-26T11:27:13.752374+00:00"}