{"created":"2023-06-26T11:01:22.702393+00:00","id":2073,"links":{},"metadata":{"_buckets":{"deposit":"d541a61a-4a85-495d-87e8-6aedeacf312a"},"_deposit":{"created_by":30,"id":"2073","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"2073"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002073","sets":["6:40"]},"author_link":["13415","13413","13419","13414","13411","13416","13412","13418","13417","13420"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-03-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"1488","bibliographicVolumeNumber":"12","bibliographic_titles":[{},{"bibliographic_title":"Nature Communications","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Strittmatter, Lisa M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Capitanchik, Charlotte"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Newman, Andrew J."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hallegger, Martina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Norman, Christine M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fica, Sebastian M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oubridge, Chris"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Luscombe, Nicholas M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ule, Jernej"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagai, Kiyoshi"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"RNA helicases remodel the spliceosome to enable pre-mRNA splicing, but their binding and mechanism of action remain poorly understood. To define helicase-RNA contacts in specific spliceosomal states, we develop purified spliceosome iCLIP (psiCLIP), which reveals dynamic helicase-RNA contacts during splicing catalysis. The helicase Prp16 binds along the entire available single-stranded RNA region between the branchpoint and 3'-splice site, while Prp22 binds diffusely downstream of the branchpoint before exon ligation, but then switches to more narrow binding in the downstream exon after exon ligation, arguing against a mechanism of processive translocation. Depletion of the exon-ligation factor Prp18 destabilizes Prp22 binding to the pre-mRNA, suggesting that proofreading by Prp22 may sense the stability of the spliceosome during exon ligation. Thus, psiCLIP complements structural studies by providing key insights into the binding and proofreading activity of spliceosomal RNA helicases.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Nature Portfolio"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/33674615","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41467-021-21745-9","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41467-021-21745-9","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2041-1723","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-14"}],"displaytype":"detail","filename":"Strittmatter-2021-psiCLIP reveals dynamic RNA.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Strittmatter-2021-psiCLIP reveals dynamic RNA","url":"https://oist.repo.nii.ac.jp/record/2073/files/Strittmatter-2021-psiCLIP reveals dynamic RNA.pdf"},"version_id":"4135d05b-ff19-4364-b513-04e437a8de7b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["40"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-05-14"},"publish_date":"2021-05-14","publish_status":"0","recid":"2073","relation_version_is_last":true,"title":["psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-06-26T11:37:22.339348+00:00"}