{"created":"2023-06-26T11:01:26.085238+00:00","id":2136,"links":{},"metadata":{"_buckets":{"deposit":"94a7ae4e-a7b0-407c-9171-d36000a82c45"},"_deposit":{"created_by":30,"id":"2136","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"2136"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002136","sets":["6:16","6:169","6:181","6:227","6:230","6:89"]},"author_link":["14081","14076","14088","14085","14079","14080","14077","14086","14083","14078","14087","14082","14084"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"9475","bibliographicVolumeNumber":"11","bibliographic_titles":[{},{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matthews, Melissa M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kim, Tae Gyun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata, Noriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Butcher, Christian"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hyun, Jaekyung"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kim, Keon Young"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Robb, Theodore"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Jheng, Siang Sheng"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Narita, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mori, Tomoari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Collins, Mary"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wolf, Matthias"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"During August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Technology Graduate University (OIST), Japan, testing for the presence of antibodies against SARS-CoV-2, the causative agent of COVID-19. We used a FDA-authorized 2-step ELISA protocol in combination with at-home self-collection of blood samples using a custom low-cost finger prick-based capillary blood collection kit. Although our survey did not find any COVID-19 seropositive individuals among the OIST cohort, it reliably detected all positive control samples obtained from a local hospital and excluded all negatives controls. We found that high serum antibody titers can persist for more than 9 months post infection. Among our controls, we found strong cross-reactivity of antibodies in samples from a serum pool from two MERS patients in the anti-SARS-CoV-2-S ELISA. Here we show that a centralized ELISA in combination with patient-based capillary blood collection using as little as one drop of blood can reliably assess the seroprevalence among communities. Anonymous sample tracking and an integrated website created a stream-lined procedure. Major parts of the workflow were automated on a liquid handler, demonstrating scalability. We anticipate this concept to serve as a prototype for reliable serological testing among larger populations.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Nature Portfolio"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/33947894","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41598-021-88850-z","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41598-021-88850-z","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-05-25"}],"displaytype":"detail","filename":"Matthews-2021-COVID-19 serological survey usin.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Matthews-2021-COVID-19 serological survey usin","url":"https://oist.repo.nii.ac.jp/record/2136/files/Matthews-2021-COVID-19 serological survey usin.pdf"},"version_id":"bf66a3e0-1642-41c0-8d2d-a5bd6f069e0a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"COVID-19","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"COVID-19 serological survey using micro blood sampling","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"COVID-19 serological survey using micro blood sampling","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["16","89","169","181","227","230"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-05-25"},"publish_date":"2021-05-25","publish_status":"0","recid":"2136","relation_version_is_last":true,"title":["COVID-19 serological survey using micro blood sampling"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-07-31T03:48:45.422497+00:00"}