{"created":"2023-06-26T11:01:30.751907+00:00","id":2237,"links":{},"metadata":{"_buckets":{"deposit":"9d8d1f5d-39d3-4d7b-84ca-96eaea3cfae4"},"_deposit":{"created_by":31,"id":"2237","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"2237"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002237","sets":["6:89"]},"author_link":["14649","14647","14646","14650","14645","14644","14648"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-07-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"858","bibliographicVolumeNumber":"4","bibliographic_titles":[{},{"bibliographic_title":"Communications Biology","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakano, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugita, Yukihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kodera, Noriyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyamoto, Sho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muramoto, Yukiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wolf, Matthias"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Noda, Takeshi"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"The single-stranded, negative-sense, viral genomic RNA (vRNA) of influenza A virus is encapsidated by viral nucleoproteins (NPs) and an RNA polymerase to form a ribonucleoprotein complex (vRNP) with a helical, rod-shaped structure. The vRNP is responsible for transcription and replication of the vRNA. However, the vRNP conformation during RNA synthesis is not well understood. Here, using high-speed atomic force microscopy and cryo-electron microscopy, we investigated the native structure of influenza A vRNPs during RNA synthesis in vitro. Two distinct types of vRNPs were observed in association with newly synthesized RNAs: an intact, helical rod-shaped vRNP connected with a folded RNA and a deformed vRNP associated with a looped RNA. Interestingly, the looped RNA was a double-stranded RNA, which likely comprises a nascent RNA and the template RNA detached from NPs of the vRNP. These results suggest that while some vRNPs keep their helical structures during RNA synthesis, for the repeated cycle of RNA synthesis, others accidentally become structurally deformed, which likely results in failure to commence or continue RNA synthesis. Thus, our findings provide the ultrastructural feature of vRNPs during RNA synthesis.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Nature Portfolio"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/34244608","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s42003-021-02388-4","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s42003-021-02388-4","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author(s)"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2399-3642","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-09-07"}],"displaytype":"detail","filename":"Nakano-2021-Ultrastructure of influenza virus.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","license_note":"CC BY 4.0\nCreative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Nakano-2021-Ultrastructure of influenza virus","url":"https://oist.repo.nii.ac.jp/record/2237/files/Nakano-2021-Ultrastructure of influenza virus.pdf"},"version_id":"abe734d4-576f-498b-9182-4e1c7b67372e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CRYSTAL-STRUCTURE","subitem_subject_scheme":"Other"},{"subitem_subject":"STRUCTURAL BASIS","subitem_subject_scheme":"Other"},{"subitem_subject":"MESSENGER-RNA","subitem_subject_scheme":"Other"},{"subitem_subject":"CAP-BINDING","subitem_subject_scheme":"Other"},{"subitem_subject":"VIRION RNA","subitem_subject_scheme":"Other"},{"subitem_subject":"POLYMERASE","subitem_subject_scheme":"Other"},{"subitem_subject":"ENDONUCLEASE","subitem_subject_scheme":"Other"},{"subitem_subject":"POLYADENYLATION","subitem_subject_scheme":"Other"},{"subitem_subject":"DYNAMICS","subitem_subject_scheme":"Other"},{"subitem_subject":"INSIGHTS","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ultrastructure of influenza virus ribonucleoprotein complexes during viral RNA synthesis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ultrastructure of influenza virus ribonucleoprotein complexes during viral RNA synthesis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["89"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-09-07"},"publish_date":"2021-09-07","publish_status":"0","recid":"2237","relation_version_is_last":true,"title":["Ultrastructure of influenza virus ribonucleoprotein complexes during viral RNA synthesis"],"weko_creator_id":"31","weko_shared_id":31},"updated":"2023-06-26T11:34:28.073749+00:00"}