{"created":"2023-06-26T11:01:33.715275+00:00","id":2291,"links":{},"metadata":{"_buckets":{"deposit":"97ec3f4f-9b4f-46ce-9181-5be17d25a3a1"},"_deposit":{"created_by":31,"id":"2291","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"2291"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002291","sets":["6:40"]},"author_link":["15223","15220","15224","15218","15221","15227","15228","15232","15231","15225","15229","15234","15226","15233","15230","15222","15219"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-09-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"18","bibliographicPageEnd":"4696.e22","bibliographicPageStart":"4680","bibliographicVolumeNumber":"184","bibliographic_titles":[{},{"bibliographic_title":"Cell","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hallegger, Martina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chakrabarti, Anob M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lee, Flora C.Y."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Lee, Bo Lim"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Amalietti, Aram G."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Odeh, Hana M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Copley, Katie E."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Rubien, Jack D."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Portz, Bede"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuret, Klara"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Huppertz, Ina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Rau, Frédérique"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Patani, Rickie"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fawzi, Nicolas L."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shorter, James"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Luscombe, Nicholas M."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ule, Jernej"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Mutations causing amyotrophic lateral sclerosis (ALS) often affect the condensation properties of RNA-binding proteins (RBPs). However, the role of RBP condensation in the specificity and function of protein-RNA complexes remains unclear. We created a series of TDP-43 C-terminal domain (CTD) variants that exhibited a gradient of low to high condensation propensity, as observed in vitro and by nuclear mobility and foci formation. Notably, a capacity for condensation was required for efficient TDP-43 assembly on subsets of RNA-binding regions, which contain unusually long clusters of motifs of characteristic types and density. These “binding-region condensates” are promoted by homomeric CTD-driven interactions and required for efficient regulation of a subset of bound transcripts, including autoregulation of TDP-43 mRNA. We establish that RBP condensation can occur in a binding-region-specific manner to selectively modulate transcriptome-wide RNA regulation, which has implications for remodeling RNA networks in the context of signaling, disease, and evolution.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/34380047","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.cell.2021.07.018","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S0092867421008771","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author(s)"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0092-8674","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1097-4172","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-09-17"}],"displaytype":"detail","filename":"1-s2.0-S0092867421008771-main.pdf","filesize":[{"value":"10.9 MB"}],"format":"application/pdf","license_note":"CC BY 4.0\nCreative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1-s2.0-S0092867421008771-main","url":"https://oist.repo.nii.ac.jp/record/2291/files/1-s2.0-S0092867421008771-main.pdf"},"version_id":"b70a6c27-5f9b-4071-9c52-e888384260a4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"RNA-binding protei","subitem_subject_scheme":"Other"},{"subitem_subject":"TDP-43","subitem_subject_scheme":"Other"},{"subitem_subject":"iCLIP","subitem_subject_scheme":"Other"},{"subitem_subject":"condensation","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA granules","subitem_subject_scheme":"Other"},{"subitem_subject":"phase separation","subitem_subject_scheme":"Other"},{"subitem_subject":"intrinsically disordered region","subitem_subject_scheme":"Other"},{"subitem_subject":"amyotrophic lateral sclerosis","subitem_subject_scheme":"Other"},{"subitem_subject":"multivalency","subitem_subject_scheme":"Other"},{"subitem_subject":"alternative polyadenylation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"TDP-43 condensation properties specify its RNA-binding and regulatory repertoire","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"TDP-43 condensation properties specify its RNA-binding and regulatory repertoire","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["40"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-09-17"},"publish_date":"2021-09-17","publish_status":"0","recid":"2291","relation_version_is_last":true,"title":["TDP-43 condensation properties specify its RNA-binding and regulatory repertoire"],"weko_creator_id":"31","weko_shared_id":31},"updated":"2023-06-26T11:33:34.231089+00:00"}