{"created":"2023-06-26T11:01:35.831923+00:00","id":2338,"links":{},"metadata":{"_buckets":{"deposit":"a0a956a1-5957-4cd7-a757-34e66d9ef14b"},"_deposit":{"created_by":31,"id":"2338","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"2338"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002338","sets":["6:74"]},"author_link":["15466","15465","15464","15462","15463"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-10-16","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{},{"bibliographic_title":"FEBS Open Bio","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Chouhan, Bhanu Pratap Singh"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Gade, Madhuri"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Martinez, Desirae"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toledo‐Patino, Saacnicteh"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Laurino, Paola"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Methionine adenosyltransferase (MAT) catalyzes the biosynthesis of S-adenosylmethionine from L-methionine and adenosine triphosphate. MAT enzymes are ancient, believed to share a common ancestor, and are highly conserved in all three domains of life. However, the sequences of archaeal MATs show considerable divergence compared to their bacterial and eukaryotic counterparts. Furthermore, the structural and functional significance of this sequence divergence are not well understood. In the present study, we employed structural analysis and ancestral sequence reconstruction (ASR) to investigate archaeal MAT divergence. We observed that the dimer interface containing the active site (which is usually well-conserved) diverged considerably between the bacterial/eukaryotic MATs and archaeal MAT. A detailed investigation of the available structures supports the sequence analysis outcome: the protein domains and subdomains of bacterial and eukaryotic MAT are more similar than those of archaea. Finally, we resurrected archaeal MAT ancestors. Interestingly, archaeal MAT ancestors show substrate specificity, which is lost during evolution. This observation supports the hypothesis of a common MAT ancestor for the three domains of life. In conclusion, we have demonstrated that archaeal MAT is an ideal system for studying an enzyme family that evolved differently in one domain compared to others while maintaining the same catalytic activity.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"John Wiley & Sons Ltd. on behalf of Federation of European Biochemical Societies"},{"subitem_publisher":"FEBS Press"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/34655277","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1002/2211-5463.13312","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://febs.onlinelibrary.wiley.com/doi/10.1002/2211-5463.13312","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author(s)"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2211-5463","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-12"}],"displaytype":"detail","filename":"2211-5463.13312 (1).pdf","filesize":[{"value":"3.5 MB"}],"format":"application/pdf","license_note":"CC BY 4.0\nCreative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"2211-5463.13312 (1)","url":"https://oist.repo.nii.ac.jp/record/2338/files/2211-5463.13312 (1).pdf"},"version_id":"e28870cf-50f6-419c-9aa6-39956535af9a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Methionine Adenosyltransferase","subitem_subject_scheme":"Other"},{"subitem_subject":"Enzyme evolution","subitem_subject_scheme":"Other"},{"subitem_subject":"Ancestral Sequence Reconstruction (ASR)","subitem_subject_scheme":"Other"},{"subitem_subject":"Catalytic interface","subitem_subject_scheme":"Other"},{"subitem_subject":"Divergence","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Implications of divergence of methionine adenosyltransferase in archaea","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Implications of divergence of methionine adenosyltransferase in archaea","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-10-19"},"publish_date":"2021-10-19","publish_status":"0","recid":"2338","relation_version_is_last":true,"title":["Implications of divergence of methionine adenosyltransferase in archaea"],"weko_creator_id":"31","weko_shared_id":31},"updated":"2023-06-26T11:32:36.647349+00:00"}