{"created":"2023-06-26T11:01:37.185734+00:00","id":2363,"links":{},"metadata":{"_buckets":{"deposit":"858121b9-5ae6-4b08-b032-3b379374ee8d"},"_deposit":{"created_by":32,"id":"2363","owners":[32],"pid":{"revision_id":0,"type":"depid","value":"2363"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002363","sets":["6:74"]},"author_link":["15722","15723","15725","15720","15719","15726","15721","15724"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-11-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"6293","bibliographicVolumeNumber":"12","bibliographic_titles":[{},{"bibliographic_title":"Nature Communications","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Testa, Andrea"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dindo, Mirco"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Rebane, Aleksander A."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nasouri, Babak"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Style, Robert W."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Golestanian, Ramin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dufresne, Eric R."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Laurino, Paola"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Living cells harvest energy from their environments to drive the chemical processes that enable life. We introduce a minimal system that operates at similar protein concentrations, metabolic densities, and length scales as living cells. This approach takes advantage of the tendency of phase-separated protein droplets to strongly partition enzymes, while presenting minimal barriers to transport of small molecules across their interface. By dispersing these microreactors in a reservoir of substrate-loaded buffer, we achieve steady states at metabolic densities that match those of the hungriest microorganisms. We further demonstrate the formation of steady pH gradients, capable of driving microscopic flows. Our approach enables the investigation of the function of diverse enzymes in environments that mimic cytoplasm, and provides a flexible platform for studying the collective behavior of matter driven far from equilibrium.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Nature Portfolio"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/34725341","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1038/s41467-021-26532-0","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.nature.com/articles/s41467-021-26532-0","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2041-1723","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-16"}],"displaytype":"detail","filename":"Testa-2021-Sustained enzymatic activity and fl.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Testa-2021-Sustained enzymatic activity and fl","url":"https://oist.repo.nii.ac.jp/record/2363/files/Testa-2021-Sustained enzymatic activity and fl.pdf"},"version_id":"95d52eb8-dfba-43c8-add4-72d8ba3eb2f9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sustained enzymatic activity and flow in crowded protein droplets","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sustained enzymatic activity and flow in crowded protein droplets","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"32","path":["74"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-16"},"publish_date":"2021-11-16","publish_status":"0","recid":"2363","relation_version_is_last":true,"title":["Sustained enzymatic activity and flow in crowded protein droplets"],"weko_creator_id":"32","weko_shared_id":32},"updated":"2023-06-26T11:32:06.443150+00:00"}