{"created":"2023-06-26T11:01:37.326325+00:00","id":2366,"links":{},"metadata":{"_buckets":{"deposit":"f25a35b3-9131-427c-b835-18b5fddf77bd"},"_deposit":{"created_by":30,"id":"2366","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"2366"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002366","sets":["6:40"]},"author_link":["15752","15753","15747","15754","15749","15750","15746","15748","15751"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-03-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"1993","bibliographicPageStart":"1985","bibliographicVolumeNumber":"144","bibliographic_titles":[{},{"bibliographic_title":"Brain","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tyzack, Giulia E"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Neeves, Jacob"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Crerar, Hamish"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Klein, Pierre"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ziff, Oliver"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taha, Doaa M"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Luisier, Raphaëlle"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Luscombe, Nicholas M"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Patani, Rickie"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"We recently described aberrantly increased cytoplasmic SFPQ intron-retaining transcripts (IRTs) and concurrent SFPQ protein mislocalization as new hallmarks of amyotrophic lateral sclerosis (ALS). However, the generalizability and potential roles of cytoplasmic IRTs in health and disease remain unclear. Here, using time-resolved deep sequencing of nuclear and cytoplasmic fractions of human induced pluripotent stem cells undergoing motor neurogenesis, we reveal that ALS-causing VCP gene mutations lead to compartment-specific aberrant accumulation of IRTs. Specifically, we identify > 100 IRTs with increased cytoplasmic abundance in ALS samples. Furthermore, these aberrant cytoplasmic IRTs possess sequence-specific attributes and differential predicted binding affinity to RNA binding proteins. Remarkably, TDP-43, SFPQ and FUS-RNA binding proteins known for nuclear-to-cytoplasmic mislocalization in ALS-abundantly and specifically bind to this aberrant cytoplasmic pool of IRTs. Our data are therefore consistent with a novel role for cytoplasmic IRTs in regulating compartment-specific protein abundance. This study provides new molecular insight into potential pathomechanisms underlying ALS and highlights aberrant cytoplasmic IRTs as potential therapeutic targets.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press on behalf of the Guarantors of Brain."}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/33693641","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1093/brain/awab078","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://academic.oup.com/brain/article/144/7/1985/6164957","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-8950","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1460-2156","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-11-17"}],"displaytype":"detail","filename":"Tyzack-2021-Aberrant cytoplasmic intron retent.pdf","filesize":[{"value":"765.9 kB"}],"format":"application/pdf","license_note":"Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Tyzack-2021-Aberrant cytoplasmic intron retent","url":"https://oist.repo.nii.ac.jp/record/2366/files/Tyzack-2021-Aberrant cytoplasmic intron retent.pdf"},"version_id":"ac056206-1520-4a52-9796-b263a94ebbdb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cytoplasmic intron retention","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"human stem cell model","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nuclear/cytoplasmic fractionation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"amyotrophic lateral sclerosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein mislocalization","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Aberrant cytoplasmic intron retention is a blueprint for RNA binding protein mislocalization in VCP-related amyotrophic lateral sclerosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Aberrant cytoplasmic intron retention is a blueprint for RNA binding protein mislocalization in VCP-related amyotrophic lateral sclerosis","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["40"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-17"},"publish_date":"2021-11-17","publish_status":"0","recid":"2366","relation_version_is_last":true,"title":["Aberrant cytoplasmic intron retention is a blueprint for RNA binding protein mislocalization in VCP-related amyotrophic lateral sclerosis"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-06-26T11:32:00.334660+00:00"}