{"created":"2023-06-26T11:01:38.180678+00:00","id":2384,"links":{},"metadata":{"_buckets":{"deposit":"1f79b2db-0989-41d1-bdcb-605d73cc7445"},"_deposit":{"created_by":30,"id":"2384","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"2384"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002384","sets":["6:42"]},"author_link":["15842"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-09-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageStart":" iyab145","bibliographicVolumeNumber":"219","bibliographic_titles":[{},{"bibliographic_title":"Genetics","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Cao, Dong"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Many circular RNAs (circRNAs) are differentially expressed in different tissues or cell types, suggestive of specific factors that regulate their biogenesis. Here, taking advantage of available mutation strains of RNA-binding proteins (RBPs) in Caenorhabditis elegans, I performed a screening of circRNA regulation in 13 conserved RBPs. Among them, loss of FUST-1, the homolog of Fused in Sarcoma (FUS), caused downregulation of multiple circRNAs. By rescue experiments, I confirmed FUST-1 as a circRNA regulator. Through RNA sequencing using circRNA-enriched samples, circRNAs targets regulated by FUST-1 were identified globally, with hundreds of them significantly altered. Furthermore, I showed that FUST-1 regulates circRNA formation with only small to little effect on the cognate linear mRNAs. When recognizing circRNA pre-mRNAs, FUST-1 can affect both exon-skipping and circRNA in the same genes. Moreover, I identified an autoregulation loop in fust-1, where FUST-1, isoform a (FUST-1A) promotes the skipping of exon 5 of its own pre-mRNA, which produces FUST-1, isoform b (FUST-1B) with different N-terminal sequences. FUST-1A is the functional isoform in circRNA regulation. Although FUST-1B has the same functional domains as FUST-1A, it cannot regulate either exon-skipping or circRNA formation. This study provided an in vivo investigation of circRNA regulation, which will be helpful to understand the mechanisms that govern circRNA formation.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press on behalf of Genetics Society of America."}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/34740247","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1093/genetics/iyab145","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://academic.oup.com/genetics/article/219/3/iyab145/6365942","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"This is a pre-copyedited, author-produced version of an article accepted for publication in Genetics following peer review. The version of record, Dong Cao, An autoregulation loop in fust-1 for circular RNA regulation in Caenorhabditis elegans, Genetics,  Volume 219, Issue 3, September 2021,  iyab145, is available online at: https://doi.org/10.1093/genetics/iyab145."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1943-2631","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":" 0016-6731","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-09-07"}],"displaytype":"detail","filename":"GENETICS-2021-304543R1_Merged_PDF.pdf","filesize":[{"value":"6.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"GENETICS-2021-304543R1_Merged_PDF","url":"https://oist.repo.nii.ac.jp/record/2384/files/GENETICS-2021-304543R1_Merged_PDF.pdf"},"version_id":"c6c76268-e0fc-4f3a-8199-c9bcc375f351"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"circular RNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FUS","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA-binding protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"autoregulation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"back splicing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"alternative splicing","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An autoregulation loop in fust-1 for circular RNA regulation in Caenorhabditis elegans","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An autoregulation loop in fust-1 for circular RNA regulation in Caenorhabditis elegans","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["42"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-11-24"},"publish_date":"2021-11-24","publish_status":"0","recid":"2384","relation_version_is_last":true,"title":["An autoregulation loop in fust-1 for circular RNA regulation in Caenorhabditis elegans"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-06-26T11:31:40.645720+00:00"}