{"created":"2023-06-26T11:01:47.877508+00:00","id":2553,"links":{},"metadata":{"_buckets":{"deposit":"70c8eef9-78f5-4c77-a9a3-d2ae88925c90"},"_deposit":{"created_by":22,"id":"2553","owners":[22],"pid":{"revision_id":0,"type":"depid","value":"2553"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002553","sets":["7:224"]},"author_link":[],"item_10006_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mbogo, Ivan","creatorNameLang":"en"}]}]},"item_10006_date_granted_11":{"attribute_name":"Degree Conferral Date","attribute_value_mlt":[{"subitem_dategranted":"2021-12-31"}]},"item_10006_degree_grantor_9":{"attribute_name":"Degree Conferrral Institution","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"Okinawa Institute of Science and Technology Graduate University"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"38005","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"Degree","attribute_value_mlt":[{"subitem_degreename":"Doctor of Philosophy"}]},"item_10006_description_7":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"The evolution of the multicellular body animals from unicellular organisms is still a significant and long-lasting subject of interest in biology. Acquisition of cell-cell adhesion with cadherin, α- and β-catenin proteins is thought to be tightly coupled with the origin of animal epithelium and consequent evolutionary thrive of animals. On the other hand, much research has shown, in a wide range of animal lineages such as bilaterians and cnidarians, that β-catenin associates with diverse intracellular proteins involved in gene transcription/translation and plays an essential role in the induction of the signalling centre (organiser) during animal embryogenesis. The pleiotropic and evolutionary conserved functions of β-catenin suggest deep evolutionary roots of the β-catenin complexes and involvement in the emergence of basic animal body plan. Recent progress in genomics has identified genes of the cell-cell adhesion complex and signalling machinery of β-catenin in genomes of early-branching animals, including Porifera (sponges) and Ctenophora(comb jellies). However, due to difficulties in applying molecular and genetic technologies in these non-model animals, the ancestral functions of β-catenin complexes remain largely to be explored.In this study, I combined structural, proteomic, and functional approaches to understand evolutionarily conserved features of the β-catenin and its associated proteins. Structural analysis suggests a unicellular origin of the basic architecture of β-catenin protein, while amino acid residues critical in adhesive properties are conserved only within animals. To analyse evolutionarily conserved functional characteristics of basal animal β-catenins, I performed transphyletic studies where the basal animal β-catenins are expressed in Xenopus embryos. A series of proteomics analyses of β-catenin-associated proteins revealed the cadherin catenin complex's deep origin and evolutionary conservation. The transphyletic function studies and detailed sequence analysis also revealed the β-catenin's organiser-inducing function of Cnidaria, Porifera, but not Ctenophora. These data suggest that the primary function of ancestral β-catenin was to play adhesive roles, and its' signalling properties were equipped later during the evolution of basal animals.","subitem_description_type":"Other"}]},"item_10006_dissertation_number_12":{"attribute_name":"Degree Referral Number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第83号"}]},"item_10006_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15102/1394.00002299","subitem_identifier_reg_type":"JaLC"}]},"item_10006_rights_13":{"attribute_name":"Copyright Information","attribute_value_mlt":[{"subitem_rights":"© 2021 The Author."}]},"item_10006_text_24":{"attribute_name":"Exam Date","attribute_value_mlt":[{"subitem_text_value":"2021-11-19"}]},"item_10006_version_type_18":{"attribute_name":"Version Format","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-12-01"}],"displaytype":"detail","filename":"FullText-Mbongo.pdf","filesize":[{"value":"11.7 MB"}],"format":"application/pdf","licensefree":"CC BY 4.0\nCreative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"FullText-Mbongo","objectType":"fulltext","url":"https://oist.repo.nii.ac.jp/record/2553/files/FullText-Mbongo.pdf"},"version_id":"f2376ecf-fa9f-439f-a5fe-9d0910f7969e"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-03-10"}],"displaytype":"detail","filename":"MbogoFinalExamAbstract.pdf","filesize":[{"value":"183.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"MbogoFinalExamAbstract","objectType":"abstract","url":"https://oist.repo.nii.ac.jp/record/2553/files/MbogoFinalExamAbstract.pdf"},"version_id":"6ee9015a-65bc-47ce-9323-d910c1e6c456"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"後生動物のベータカテニン多機能化の進化に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"後生動物のベータカテニン多機能化の進化に関する研究","subitem_title_language":"ja"},{"subitem_title":"The Evolution of Dual Functionality of β-catenin in Metazoans","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"22","path":["224"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-02-14"},"publish_date":"2022-02-14","publish_status":"0","recid":"2553","relation_version_is_last":true,"title":["後生動物のベータカテニン多機能化の進化に関する研究"],"weko_creator_id":"22","weko_shared_id":-1},"updated":"2023-08-31T05:47:07.940075+00:00"}