{"created":"2023-06-26T11:01:55.176902+00:00","id":2675,"links":{},"metadata":{"_buckets":{"deposit":"dce33d20-bd6e-4a23-a963-7178aae83d5d"},"_deposit":{"created_by":30,"id":"2675","owners":[30],"pid":{"revision_id":0,"type":"depid","value":"2675"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002675","sets":["6:171"]},"author_link":["17297","17298"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03-28","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"162","bibliographicPageStart":"156","bibliographicVolumeNumber":"606","bibliographic_titles":[{},{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamazaki, Yuta"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kono, Keiko"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Plasma membrane damage and repair frequently happen in cells. A critical process underlying plasma membrane repair is to redirect repair factors, such as protein kinase C and the exocyst complex, from the polarized site to the damage site. However, the mechanism underlying the repair factor delivery to the damage site remains unknown. Here, we demonstrate that clathrin-mediated trafficking of repair factors is involved in plasma membrane/cell wall repair in budding yeast. Using laser-induced plasma membrane/cell wall damage assay, we identified phospholipid flippases, Lem3-Dnf1/Dnf2 and Cdc50-Drs2, as essential clathrin cargos for plasma membrane/cell wall repair. We found that flippase impairment significantly compromised the recruitment of exocyst Exo70 to the damage site. In contrast, the recruitment of protein kinase C (Pkc1) was only mildly compromised. Taken together, clathrin-mediated trafficking of the phospholipid flippases is critical for the recruitment of exocyst to the damage site. Mechanisms to redirect exocyst via the clathrin and flippase-mediated pathways may be a general feature of effective plasma membrane repair in polarized cells.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/35358840","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.bbrc.2022.03.129","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.sciencedirect.com/science/article/pii/S0006291X22004740?via%3Dihub","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2022 The Author(s)."}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-05"}],"displaytype":"detail","filename":"1-s2.0-S0006291X22004740-main.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","license_note":"Creative Commons Attribution-NonCommercial 4.0 International(https://creativecommons.org/licenses/by-nc/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1-s2.0-S0006291X22004740-main","url":"https://oist.repo.nii.ac.jp/record/2675/files/1-s2.0-S0006291X22004740-main.pdf"},"version_id":"c222da08-3571-45cc-b59d-02be627f800b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Plasma membrane repair","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Clathrin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phospholipid flippase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Exocyst","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Exo70","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Budding yeast","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Clathrin-mediated trafficking of phospholipid flippases is required for local plasma membrane/cell wall damage repair in budding yeast","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Clathrin-mediated trafficking of phospholipid flippases is required for local plasma membrane/cell wall damage repair in budding yeast","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"30","path":["171"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-07-05"},"publish_date":"2022-07-05","publish_status":"0","recid":"2675","relation_version_is_last":true,"title":["Clathrin-mediated trafficking of phospholipid flippases is required for local plasma membrane/cell wall damage repair in budding yeast"],"weko_creator_id":"30","weko_shared_id":30},"updated":"2023-06-26T11:26:41.701914+00:00"}