{"created":"2023-06-26T11:01:56.378905+00:00","id":2695,"links":{},"metadata":{"_buckets":{"deposit":"0aadca67-c6a4-4095-a74d-92cce0bb6fb8"},"_deposit":{"created_by":31,"id":"2695","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"2695"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002695","sets":["6:66"]},"author_link":["17427","17426","17428","17429"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-04-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicVolumeNumber":"12","bibliographic_titles":[{},{"bibliographic_title":"Open Biology","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Xu, Xingya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanai, Ryuta"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Li"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagida, Mitsuhiro"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Cohesin holds sister chromatids together and is cleaved by separase/Cut1 to release DNA during the transition from mitotic metaphase to anaphase. The cohesin complex consists of heterodimeric structural maintenance of chromosomes (SMC) subunits (Psm1 and Psm3), which possess a head and a hinge, separated by long coiled coils. Non-SMC subunits (Rad21, Psc3 and Mis4) bind to the SMC heads. Kleisin/Rad21's N-terminal domain (Rad21-NTD) interacts with Psm3's head-coiled coil junction (Psm3-HCJ). Spontaneous mutations that rescued the cleavage defects in temperature-sensitive (ts) separase mutants were identified in the interaction interface, but the underlying mechanism is yet to be understood. Here, we performed site-directed random mutagenesis to introduce single amino acid substitutions in Psm3-HCJ and Rad21-NTD, and then identified 300 mutations that rescued the cohesin-releasing defects in a separase ts mutant. Mutational analysis indicated that the amino acids involved in hydrophobic cores (which may be in close contact) in Psm3-HCJ and Rad21-NTD are hotspots, since 80 mutations (approx. 27%) were mapped in these locations. Properties of these substitutions indicate that they destabilize the interaction between the Psm3 head and Rad21-NTD. Thus, they may facilitate sister chromatid separation in a cleavage-independent way through cohesin structural re-arrangement.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"The Royal Society"}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/35472286","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1098/rsob.210275","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://royalsocietypublishing.org/doi/10.1098/rsob.210275","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2022 The Author(s)"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2046-2441","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-07-06"}],"displaytype":"detail","filename":"rsob.210275_Redacted.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","license_note":"CC BY 4.0\nCreative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"rsob.210275_Redacted","url":"https://oist.repo.nii.ac.jp/record/2695/files/rsob.210275_Redacted.pdf"},"version_id":"b6dead44-6094-4ce8-85cb-a3cb462e4a9f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"saturation mutagenesis","subitem_subject_scheme":"Other"},{"subitem_subject":"suppressor screen","subitem_subject_scheme":"Other"},{"subitem_subject":"cohesin","subitem_subject_scheme":"Other"},{"subitem_subject":"hydrophobic core","subitem_subject_scheme":"Other"},{"subitem_subject":"coiled coils","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Single amino acid substitutions in hydrophobic cores at a head-coiled coil junction region of cohesin facilitate its release of DNA during anaphase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Single amino acid substitutions in hydrophobic cores at a head-coiled coil junction region of cohesin facilitate its release of DNA during anaphase","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["66"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-07-06"},"publish_date":"2022-07-06","publish_status":"0","recid":"2695","relation_version_is_last":true,"title":["Single amino acid substitutions in hydrophobic cores at a head-coiled coil junction region of cohesin facilitate its release of DNA during anaphase"],"weko_creator_id":"31","weko_shared_id":31},"updated":"2023-06-26T11:26:24.145943+00:00"}