{"created":"2023-06-26T11:01:58.272968+00:00","id":2732,"links":{},"metadata":{"_buckets":{"deposit":"c8e9fb18-b84b-41ce-8cc0-4d2347fae22c"},"_deposit":{"created_by":31,"id":"2732","owners":[31],"pid":{"revision_id":0,"type":"depid","value":"2732"},"status":"published"},"_oai":{"id":"oai:oist.repo.nii.ac.jp:00002732","sets":["6:64"]},"author_link":["17592","17591"],"item_10001_biblio_info_7":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-07-31","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{},{"bibliographic_title":"Journal of Cellular Biochemistry","bibliographic_titleLang":"en"}]}]},"item_10001_creator_3":{"attribute_name":"Author","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Baeken, Marius W."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokobayashi, Yohei"}],"nameIdentifiers":[{}]}]},"item_10001_description_5":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"EGFP (enhanced green fluorescent protein) is one of the most common tools used in life sciences, including research focusing on proteostasis. Here we report that ERN1 (endoplasmic reticulum to nucleus signaling 1), which is upregulated by UPR (unfolded protein response), targets an RNA hairpin loop motif in EGFP mRNA. A silent mutation introduced into EGFP mRNA abolished the ERN1-dependent mRNA decay. Therefore, experiments that employ EGFP as a reporter gene in studies that involve upregulation of the UPR pathway should be interpreted carefully, and a mutant devoid of the ERN1 target motif may be more suitable for such studies.","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Wiley Periodicals LLC."}]},"item_10001_relation_13":{"attribute_name":"PubMedNo.","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/35908204","subitem_relation_type_select":"PMID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1002/jcb.30314","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"Related site","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://onlinelibrary.wiley.com/doi/10.1002/jcb.30314","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2022 The Authors. "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0730-2312","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1097-4644","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"Author's flag","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-08-04"}],"displaytype":"detail","filename":"Baeken-2022-Identification of an ERN1 target s.pdf","filesize":[{"value":"5.8 MB"}],"format":"application/pdf","license_note":"CC BY-NC 4.0\nCreative Commons Attribution-NonCommercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Baeken-2022-Identification of an ERN1 target s","url":"https://oist.repo.nii.ac.jp/record/2732/files/Baeken-2022-Identification of an ERN1 target s.pdf"},"version_id":"3b6f519a-f327-4b7f-afde-ad0424996be5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"EGFP","subitem_subject_scheme":"Other"},{"subitem_subject":"ERN1","subitem_subject_scheme":"Other"},{"subitem_subject":"IRE1","subitem_subject_scheme":"Other"},{"subitem_subject":"proteostasis","subitem_subject_scheme":"Other"},{"subitem_subject":"unfolded protein response","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of an ERN1 target site within EGFP mRNA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of an ERN1 target site within EGFP mRNA","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"31","path":["64"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-08-04"},"publish_date":"2022-08-04","publish_status":"0","recid":"2732","relation_version_is_last":true,"title":["Identification of an ERN1 target site within EGFP mRNA"],"weko_creator_id":"31","weko_shared_id":31},"updated":"2023-06-26T11:25:27.594986+00:00"}