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Analysis of the Sequence Preference of Saporin by Deep Sequencing
https://oist.repo.nii.ac.jp/records/2761
https://oist.repo.nii.ac.jp/records/276140b8bdab-7d1b-4d32-91e8-36ad9ec9eaa7
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Hauf-2022-Analysis of the Sequence Preference_am (5.5 MB)
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Hauf-2024-Analysis of the Sequence Preference.pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| PubDate | 2022-08-22 | |||||||||||
| Title | ||||||||||||
| Title | Analysis of the Sequence Preference of Saporin by Deep Sequencing | |||||||||||
| Language | en | |||||||||||
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| Language | eng | |||||||||||
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| Language | en | |||||||||||
| Subject Scheme | Other | |||||||||||
| Subject | Adenine | Assays | Crystal cleavage | Genetics | Polyacrylamide gel electrophoresis | |||||||||||
| Resource Type | ||||||||||||
| Resource Type Identifier | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| Resource Type | journal article | |||||||||||
| Access Right | ||||||||||||
| Access Rights | open access | |||||||||||
| Access Rights URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| Author |
Hauf, Samuel
× Hauf, Samuel
× Rotrattanadumrong, Rachapun
× Yokobayashi, Yohei
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| Bibliographic Information |
en : ACS Chemical Biology Volume Number 17, Issue Number 9, p. 2619-2630, Issue Date 2022-08-15 |
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| Abstract | ||||||||||||
| Description Type | Abstract | |||||||||||
| Description | Ribosome-inactivating proteins (RIPs) are RNA:adenosine glycosidases that inactivate eukaryotic ribosomes by depurinating the sarcin-ricin loop (SRL) in 28S rRNA. The GAGA sequence at the top of the SRL or at the top of a hairpin loop is assumed to be their target motif. Saporin is a RIP widely used to develop immunotoxins for research and medical applications, but its sequence specificity has not been investigated. Here, we combine the conventional aniline cleavage assay for depurinated nucleic acids with high-throughput sequencing to study sequence-specific depurination of oligonucleotides caused by saporin. Our data reveal the sequence preference of saporin for different substrates and show that the GAGA motif is not efficiently targeted by this protein, neither in RNA nor in DNA. Instead, a preference of saporin for certain hairpin DNAs was observed. The observed sequence-specific activity of saporin may be relevant to antiviral or apoptosis-inducing effects of RIPs. The developed method could also be useful for studying the sequence specificity of depurination by other RIPs or enzymes. | |||||||||||
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| Publisher | American Chemical Society | |||||||||||
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| Source Identifier Type | PISSN | |||||||||||
| Source Identifier | 1554-8929 | |||||||||||
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| Source Identifier Type | EISSN | |||||||||||
| Source Identifier | 1554-8937 | |||||||||||
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| Relation Type | isIdenticalTo | |||||||||||
| Identifier Type | PMID | |||||||||||
| Related Identifier | 35969718 | |||||||||||
| item_10001_relation_14 | ||||||||||||
| Relation Type | isIdenticalTo | |||||||||||
| Identifier Type | DOI | |||||||||||
| Related Identifier | 10.1021/acschembio.2c00531 | |||||||||||
| Rights | ||||||||||||
| Rights | © 2022 The Author(s) | |||||||||||
| Rights | ||||||||||||
| Rights Resource | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||||||||
| Rights | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International | |||||||||||
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| Relation Type | isIdenticalTo | |||||||||||
| Identifier Type | URI | |||||||||||
| Related Identifier | https://pubs.acs.org/doi/10.1021/acschembio.2c00531 | |||||||||||
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| Relation Type | isReplacedBy | |||||||||||
| Identifier Type | URI | |||||||||||
| Related Identifier | https://pubs.acs.org/doi/10.1021/acschembio.3c00733 | |||||||||||
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| Version Type | VoR | |||||||||||
| Version Type Resource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
